PepTivator EBV BZLF1, human

PepTivator EBV BZLF1, human

PepTivator
®
EBV BZLF1 is a peptide pool that consists mainly of 15-mer peptides with 11 amino acid (aa) overlap, covering the complete sequence of the Epstein-Barr virus BZLF1 protein (UniProt ID: P03206).
PepTivator peptide pools have been developed for the efficient
in vitro
stimulation of antigen–specific CD4
+
and CD8
+
T cells as peptides of 15-aa length with 11-aa overlap represent an optimized solution for stimulating both CD4
+
and CD8
+
T cells in various applications.
1
Quantitative, phenotypical, or functional analysis of EBV BZLF1–specific T cell immunity can provide important information on the natural course of immune responses in healthy or immunocompromised individuals.
PepTivator EBV BZLF1-HT is a peptide pool lyophilized in the wells of a 96-well plate, allowing stimulation and analysis of cells directly on the plate.
The convenient 96-well format, composed of 12 individually removable strips of 8 wells each allows for easy and flexible experimental set-up, different antigens can be combined by assembling different strips. A control can also be added easily by using strips from the Control plate (12x8).

Background information

Epstein-Barr virus (EBV) is a human γ-herpesvirus with B cell growth–transforming ability and lymphomagenic potential. More than 90% of human adults are infected with EBV. Usually the primary infection is asymptomatic, but some individuals develop an infectious mononucleosis, a self-limiting lymphoproliferative disease. BZLF1 activates a promoter of an EBV gene and plays a key role in the switch from latent to productive infection of EBV. BZLF1-specific CD4
+
and CD8
+
T cells have been found in EBV-infected individuals.
2–4

Downstream applications

The
in vitro
stimulation of EBV BZLF1–specific CD4
+
and CD8
+
T cells with PepTivator EBV BZLF1 causes the secretion of effector cytokines and the upregulation of activation markers, which then allow the detection and isolation of EBV BZLF1–specific T cells
5
:
  • Detection and analysis of EBV BZLF1–specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
  • Isolation of viable EBV BZLF1–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation of EBV BZLF1–specific CD4+ and CD8+ effector/memory T cells from naive T cell populations for research on immunotherapy and vaccination.
  • Pulsing of antigen-presenting cells, for example, for research on dendritic cell vaccination.
Product options: 3

Product information

Size
Order no.
Price

PepTivator EBV BZLF1 - premium grade, human

  • For research use only
for
stimulation of
10
8
cells
130-093-611
-

PepTivator EBV BZLF1 - premium grade, human

  • For research use only
for
stimulation of
10
9
cells
130-093-612
-

PepTivator EBV BZLF1 (HT) - premium grade, human

  • For research use only
for 96
tests
130-098-239
-

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