StemMACS™ CHIR99021

StemMACS™ CHIR99021

StemMACS™ CHIR99021 is a selective small molecule GSK3 inhibitor that activates Wnt signaling.

Background information

StemMACS™ CHIR99021 is a highly selective inhibitor of glycogen synthase kinase 3 (GSK-3), a crucial regulator of the Wnt signaling pathway. The aminopyridine CHIR99021 inhibits both GSK-3 isoforms, GSK-3α (IC50 10 nM) and GSK-3β (IC50 6.7 nM). Unlike other GSK-3 inhibitors, it does not cross-react with cyclin-dependent kinases (CDKs). Activation of Wnt signaling via CHIR99021-mediated GSK-3 inhibition is widely used to modulate pluripotent stem cell differentiation and self-renewal.

Applications

Published applications for CHIR99021 include:
  • Naïve, ground state culture of murine ES and iPS cells using 2i conditions1
  • Naïve, ground state culture of rat ES and iPS cells using 3i conditions2
  • Naïve, ground state culture of human ES and iPS cells3
  • Differentiation of human ES and iPS cells into cardiomyocytes4
  • Differentiation of human ES and iPS cells into skeletal muscle5
  • Differentiation of human ES and iPS cells into definitive endoderm6
  • Chemical Reprogramming of mouse fibroblasts5
Product options: 3

Product information

Size
Order no.
Price

StemMACS CHIR99021

  • For research use only
2 mg
130-103-926
-

StemMACS CHIR99021

  • For research use only
5×2 mg
130-104-172
-

StemMACS CHIR99021 in Solution

  • For research use only
2 mg
130-106-539
-

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