StemMACS™ CHIR99021

StemMACS™ CHIR99021

StemMACS™ CHIR99021 is a selective small molecule GSK3 inhibitor that activates Wnt signaling.

Background information

StemMACS™ CHIR99021 is a highly selective inhibitor of glycogen synthase kinase 3 (GSK-3), a crucial regulator of the Wnt signaling pathway. The aminopyridine CHIR99021 inhibits both GSK-3 isoforms, GSK-3α (IC50 10 nM) and GSK-3β (IC50 6.7 nM). Unlike other GSK-3 inhibitors, it does not cross-react with cyclin-dependent kinases (CDKs). Activation of Wnt signaling via CHIR99021-mediated GSK-3 inhibition is widely used to modulate pluripotent stem cell differentiation and self-renewal.


Published applications for CHIR99021 include:
  • Naïve, ground state culture of murine ES and iPS cells using 2i conditions1
  • Naïve, ground state culture of rat ES and iPS cells using 3i conditions2
  • Naïve, ground state culture of human ES and iPS cells3
  • Differentiation of human ES and iPS cells into cardiomyocytes4
  • Differentiation of human ES and iPS cells into skeletal muscle5
  • Differentiation of human ES and iPS cells into definitive endoderm6
  • Chemical Reprogramming of mouse fibroblasts5
  • Selected References

    1. Solbrant, S. et al. (2017) Generation of high-purity human ventral midbrain dopaminergic progenitors for in vitro maturation and intracerebral transplantation Nat. Protoc. 12(9): 1962-1979
    2. Ying, Q. L. et al. (2008) The ground state of embryonic stem cell self-renewal. Nature 453: 519-523
    3. Li et al. (2008) Germline competent embryonic stem cells derived from rat blastocysts. Cell 135: 1299-1310
    4. Gafni, O. et al. (2013) Derivation of novel human ground state naive pluripotent stem cells. Nature 504: 282-286
    5. Lian, X. et al. (2012) Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc. Natl. Acad. Sci. U.S.A. 109(27): E1848-1857
    6. Borchin, B. et al. (2013) Derivation and FACS-mediated purification of PAX3+/PAX7+ skeletal muscle precursors from human pluripotent stem cells. Stem Cell Reports 1: 620-631
    7. Teo, A. K. K. et al. (2014) Comparable generation of activin-induced definitive endoderm via additive Wnt or BMP signaling in absence of serum. Stem Cell Reports 3: 5-14
    8. Hou, P. et al. (2013) Pluripotent stem cells induced from mouse somatic cells by small-molecule compounds. Science 341(6146): 651-654
  • Brochures and posters

Product options: 3

StemMACS CHIR99021

2 mg

StemMACS CHIR99021

5×2 mg

StemMACS CHIR99021 in Solution

2 mg