StemMACS™ NeuroG2 mRNA, human

StemMACS™ NeuroG2 mRNA, human

StemMACS™ NeuroG2 mRNA encodes the human transcription factor Neurogenin 2 for transient transfection.

Applications

  • Neuronal differentiation of pluripotent stem cells1,2
  • Conversion of fibroblasts into induced neuronal (iN) cells3

Background information

Neurogenin 2, encoded by the human NeuroG2 (NGN2) gene, is a neural-specific basic helix-loop-helix (bHLH) transcription factor that regulates specification of neuronal fate in ectodermal cells. Neurogenin 2 is expressed in neural progenitor cells within the developing central and peripheral nervous systems. In addition, it plays a role in the differentiation and survival of midbrain dopaminergic neurons. In pluripotent stem cell research, ectopic expression of Neurogenin 2 has been used to promote neuronal differentiation of ES or iPS cells
1,2
and to convert fibroblasts into induced neuronal (iN) cells
3
.

Detailed procedure

For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.

Quality description

StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.
StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.
Product options: 1

Product information

Size
Order no.
Price

StemMACS NeuroG2 mRNA, human

  • For research use only
20 µg
130-104-383
-

Related products

3 products available