StemMACS™ Myt1l mRNA, human

StemMACS™ Myt1l mRNA, human

StemMACS™ Myt1l mRNA encoding the human transcription factor Myt1l for transient transfection.

Applications

  • Neuronal differentiation of pluripotent stem cells1,2
  • Conversion of fibroblasts into induced neuronal (iN) cells3

Background information

Myelin transcription factor-like 1 protein (Myt1l) is a pan-neural transcription factor that regulates neuronal differentiation. In pluripotent stem cell research, ectopic expression of Myt1l in combination with other transcription factors (e.g., Brn2, Mash1 (Ascl1)) has been used to induce neural differentiation.

Detailed procedure

For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.

Quality description

StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.
StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.
  • Selected References

    1. Pang et al. (2011) Induction of human neuronal cells by defined transcription factors. Nature 476: 220-223
    2. Vierbuchen et al. (2010) Direct conversion of fibroblasts to functional neurons by defined factors. Nature 463: 1035-1041
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Product information

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StemMACS Myt1l mRNA, human

  • For research use only
20 µg
130-104-379
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