StemMACS™ Mash1 (Ascl1) mRNA, human

StemMACS™ Mash1 (Ascl1) mRNA, human

StemMACS™ Mash1 (Ascl1) mRNA encodes the human transcription factor Mash1 for transient transfection.

Applications

  • Neuronal differentiation of pluripotent stem cells1,2
  • Conversion of fibroblasts into induced neuronal (iN) cells3,4

Background information

The basic helix-loop-helix (BHLH) transcription factor Mash1(Ascl1) dimerizes with other BHLH family members to activate transcription via recognition of the E-box motif (5’-CANNTG-3’). In pluripotent stem cell biology, Mash1 plays a role in neuronal commitment and differentiation, e.g. in the generation of olfactory and autonomic neurons.

Detailed procedure

For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.

Quality description

StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.
StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.
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