StemMACS™ eGFP mRNA

StemMACS™ eGFP mRNA

StemMACS™ eGFP mRNA encodes enhanced green fluorescent protein (eGFP) for transient transfection.

Applications

  • Positive control for mRNA transfections
  • Optimization of mRNA transfection protocols

Background information

StemMACS™ eGFP mRNA was designed for transient expression of the eGFP protein after transfection. Fluorescence of the eGFP protein can be easily detected by fluorescence microscopy or flow cytometry. StemMACS™ eGFP mRNA is therefore ideal for monitoring and optimizing transfection efficiency and is recommended as a positive control for mRNA transfections.

Detailed procedure

For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.

Quality description

StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.
StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.
Product options: 1

Product information

Size
Order no.
Price

StemMACS eGFP mRNA

  • For research use only
20 µg
130-101-114
-

Related products

3 products available