Mouse VEGF

Mouse VEGF

VEGF stands for vascular endothelial growth factor, also termed VEGF-A. Several alternatively spliced isoforms have been identified in mice. Mouse VEGF (164 aa) is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse VEGF (164 aa) can be used for a variety of applications, including:
  • Proliferation of endothelial cells.
  • Promotion of endothelial cell migration.
  • Chemo-attractant function, inducing migration of monocytes and osteoblasts.
  • Increasing the release of von Willebrand factor from endothelial cells and metallo-proteinases activity.

Background information

Vascular endothelial growth factor (VEGF), a disulfide-linked homodimer, also known as VEGF-A, belongs to the platelet-derived growth factor superfamily. VEGF is secreted by vascular smooth muscle cells upon hypoxic conditions and promotes angiogenesis and vasculogenesis, vascular permeability, and inhibition of apoptosis, through the binding to two cell surface receptors, VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1), and other co-receptors, which are expressed mainly on endothelial cells and immune cells. VEGFR2 mediates almost all observed endothelial responses to VEGF, while Neuropilin-1 acts as co-receptor for the VEGF 165 aa isoform and enhances its binding to VEGFR2 and its biological activity. The VEGF/VEGFR system supports initiation of inflammation, inducing migration of monocytes and macrophages, but also acts on neurons and kidney epithelial cells. Moreover, VEGF contributes to tumor growth and metastasis formation, and is crucial during embryonic development and wound healing. Alteration in VEGF/VEGFR pathways have been associated with diseases, such as cancer, age-related macular degeneration, preeclampsia, rheumatoid arthritis, and neuronal disorders, such as amyotrophic lateral sclerosis. Several isoforms are generated as a result of alternative splicing, including the soluble isoforms VEGF 121 aa and VEGF 165 aa in human, and VEGF 164 aa isoform in mouse.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Biological activity

  • Proliferation of human umbilical vein endothelial cells
  • research grade: ≥ 5×
    10
    5
    U/mg
Product options: 2

Product information

Size
Order no.
Price

Mouse VEGF (164 aa), research grade

  • Insect cells
  • For research use only
5 µg
130-094-086
-

Mouse VEGF (164 aa), research grade

  • Insect cells
  • For research use only
20 µg
130-094-087
-