Mouse LIF

Mouse LIF

LIF stands for leukemia inhibitory factor. Mouse LIF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse LIF can be used for a variety of applications including:
  • Maintenance of self-renewal and pluripotency in conventional mouse ESC cultures.

Background information

LIF is a pleiotropic cytokine, which is critically involved in embryonic development and blastocyst implantation. LIF belongs to the interleukin 6 family and functions through the gp130 activation of STAT3. In mice LIF is a key factor that prevents embryonic stem cells (ESC) to differentiate. Additionally, LIF affects hematopoiesis, neural development, bone and energy metabolism, and inflammation.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Inhibition of M1 cells
  • premium grade: ≥ 2×
    10
    6
    U/mg
    (typical activity: ≥ 2.5×
    10
    6
    U/mg
    )
  • research grade: ≥ 1×
    10
    6
    U/mg
Product options: 6

Product information

Size
Order no.
Price

Mouse LIF, research grade

  • E. coli
  • For research use only
10 µg
130-095-772
-

Mouse LIF, research grade

  • E. coli
  • For research use only
25 µg
130-095-775
-

Mouse LIF, premium grade

  • E. coli
  • For research use only
10 µg
130-095-777
-

Mouse LIF, premium grade

  • E. coli
  • For research use only
25 µg
130-095-778
-

Mouse LIF, premium grade

  • E. coli
  • For research use only
100 µg
130-095-779
-

Mouse LIF, premium grade

  • E. coli
  • For research use only
10×100 µg
130-099-895
-