Mouse GM-CSF

Mouse GM-CSF

GM-CSF stands for granulocyte macrophage colony-stimulating factor. Mouse GM-CSF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse GM-CSF can be used for a variety of applications, including:
  • Induction of colony formation of granulocyte/macrophage progenitors in semi-solid medium.
  • In vitro generation of DCs from bone marrow or maturation of CD11c+ splenocytes.
  • Generation of antigen-presenting (DC-like) cells in primary brain cell culture.

Background information

GM-CSF is a hematopoietic growth factor, which is essential for proliferation and development of granulocyte and monocyte/macrophage progenitors. It also functions as a growth factor for erythroid and megakaryocytic precursor cells in conjunction with erythropoietin. GM-CSF is secreted by various cell types including T cells, macrophages, endothelial cells, and fibroblasts in response to inflammatory stimuli and cytokines. In addition, GM-CSF strongly chemoattracts neutrophils and eosinophils and enhances the effector functions of neutrophils and macrophages.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of FDC-P1 cells (NIBSC 91/658)
  • premium grade: ≥ 5×
    10
    7
    U/mg
  • research grade: ≥ 1×
    10
    7
    U/mg
Product options: 6

Product information

Size
Order no.
Price

Mouse GM-CSF, research grade

  • E. coli
  • For research use only
10 µg
130-094-043
-

Mouse GM-CSF, research grade

  • E. coli
  • For research use only
25 µg
130-095-746
-

Mouse GM-CSF, premium grade

  • E. coli
  • For research use only
10 µg
130-095-742
-

Mouse GM-CSF, premium grade

  • E. coli
  • For research use only
25 µg
130-095-793
-

Mouse GM-CSF, premium grade

  • E. coli
  • For research use only
100 µg
130-095-739
-

Mouse GM-CSF, premium grade

  • E. coli
  • For research use only
1000 µg
130-095-735
-