Mouse FGF-8b

Mouse FGF-8b

FGF-8b stands for fibroblast growth factor 8b, originally identified as androgen-induced growth factor. Mouse FGF-8b is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse FGF-8b can be used for a variety of applications, including:
  • In vitro expansion of embryonic stem cell–derived neural progenitor cells.
  • Survival of neural precursor cells and differentiation into astroglial cells.
  • Stimulation of osteoblast proliferation.
  • Proliferation of endothelial cells.
  • Protection of hippocampal cultures from oxidative stress.
  • Proliferation of myogenic cells.
  • Tumor growth studies of prostate and breast cancer.

Background information

Fibroblast growth factor 8 (FGF-8) is a heparin-binding protein, which has mitogenic and transforming activity. Eight secreted FGF-8 isoforms (FGF-8a to h) have been identified in mice, whereas four have been described in humans (a, b, e, and f). FGF-8b appears to be the predominant form and has been shown to have the most important oncogenic transforming capacity. FGF-8 exerts a pivotal role in embryogenesis. It is expressed during gastrulation and influences brain, limb, heart, and facial development in the mouse embryo. In addition, FGF-8 was shown to stimulate osteoblast proliferation.The amino acid sequence of mouse FGF8-b shares 100% identity with human FGF-8b.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of 3T3 cells
  • premium grade: ≥ 2×
    10
    4
    U/mg
  • research grade: ≥ 1×
    10
    4
    U/mg
Product options: 6

Product information

Size
Order no.
Price

Mouse FGF-8b, research grade

  • E. coli
  • For research use only
10 µg
130-096-100
-

Mouse FGF-8b, research grade

  • E. coli
  • For research use only
25 µg
130-096-101
-

Mouse FGF-8b, premium grade

  • E. coli
  • For research use only
10 µg
130-096-102
-

Mouse FGF-8b, premium grade

  • E. coli
  • For research use only
25 µg
130-096-103
-

Mouse FGF-8b, premium grade

  • E. coli
  • For research use only
100 µg
130-096-104
-

Mouse FGF-8b, premium grade

  • E. coli
  • For research use only
1000 µg
130-096-105
-

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