Human SDF-1α

Human SDF-1α

SDF-1 stands for stromal cell-derived factor-1, also termed CXCL12 or CXC motif chemokine 12. Human SDF-1α is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human SDF-1α may be used for a variety of applications, including:
  • Migration and homing assays.
  • In vitro angiogenesis assays (cell outgrowth, proliferation, and tube formation).
  • Adhesion assays.
  • Studies on tumorigenesis, organogenesis, neuronal development, and autoimmune diseases.

Alternative Names

CXCL12

Background information

Stromal cell–derived factor-1 (SDF-1), also called CXCL12 or CXC motif chemokine 12, is a small cytokine with chemotactic activity. The sequence of SDF-1 is highly conserved among the species. Mouse SDF-1 differs only in one amino acid from human SDF-1 and therefore shows cross-reactivity. SDF-1 binds to the G-protein-coupled seven-transmembrane receptor CXCR4 and induces a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. CXCR7 has been identified as another seven-transmembrane receptor for SDF-1, suggesting a role for SDF-1 in several aspects of tumorigenesis.
In vitro
, SDF-1 is a potent chemoattractant for T lymphocytes and monocytes, but not for neutrophils. Furthermore SDF-1 acts as a chemoattractant for CD34
+
human hematopoietic progenitor cells
in vivo
and
in vitro
, as well as for mesenchymal stem cells
in vitro
. SDF-1 is implicated in neuronal migration, neural development, brain inflammation, and tumor growth
in vivo
.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Biological activity

  • Transwell
    ®
    chemotaxis assay using U-937 cells
Product options: 4

Product information

Size
Order no.
Price

Human SDF-1α, research grade

  • E. coli
  • For research use only
10 µg
130-093-996
-

Human SDF-1α, research grade

  • E. coli
  • For research use only
25 µg
130-096-137
-

Human SDF-1α, research grade

  • E. coli
  • For research use only
100 µg
130-093-997
-

Human SDF-1α, research grade

  • E. coli
  • For research use only
1000 µg
130-093-998
-