Human SCF

Human SCF

SCF stands for stem cell factor, also termed c-kit ligand, mast cell growth factor (MGF), or steel factor (SLF). Human SCF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human SCF can be used for a variety of applications, including:
  • Stimulation of proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures.
  • In vitro expansion of CD34+ hematopoietic progenitor cells.
  • Differentiation of ES-derived cells towards the hematopoietic lineage.
  • Mast cell differentiation and maintenance.
  • Mast cell chemotaxis assays.

Alternative Names

c-kit ligand, steel factor, MGF

Background information

SCF is a hematopoietic growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and progenitor cells. Besides its pivotal role in mast cell development, SCF acts as a potent mast cell chemoattractant and upregulates mast cell adhesion and migration. SCF signals through the c-kit receptor (CD117) and exists in two forms; cell surface bound SCF and soluble SCF. The secreted soluble form of SCF is produced by the proteolytic processing of the cell surface anchored precursor molecule.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of TF-1 cells (NIBSC 91/682)
  • premium grade: ≥ 4×
    10
    5
    U/mg
    (typical activity: ≥ 9×
    10
    5
    U/mg
    )
  • research grade: ≥ 2×
    10
    5
    U/mg
  • Selected References

    1. Laurenti, E. et al. (2015) CDK6 levels regulate quiescence exit in human hematopoietic stem cells. Cell Stem Cell 16(3): 302-313
    2. Narla, A. et al. (2011) Dexamethasone and lenalidomide have distinct functional effects on erythropoiesis. Blood 118(8): 2296-2304
    3. Velardi, E. et al. (2014) Sex steroid blockade enhances thymopoiesis by modulating Notch signaling. J. Exp. Med. 211(12): 2341-2349
    4. Kitumura, T. et al. (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334
    5. Dighe N. et al. (2014) Long-term reproducible expression in human fetal liver hematopoietic stem cells with a UCOE-based lentiviral vector. PLoS One 9(8): e104805
    6. Dussiau C. et al. (2015) Targeting IRAK1 in T-cell acute lymphoblastic leukemia. Oncotarget 6: 18956-18965
    7. Brault, J. et al. (2014)
      Optimized generation of functional neutrophils and macrophages from patient-specific induced pluripotent stem cells:
      ex vivo
      models of X
      0
      -linked, AR22
      0
      - and AR47
      0
      - chronic granulomatous diseases.
      Biores Open Access 3(6): 311-326
    8. Steinleitner, K. et al. (2012) EVI1 and MDS1/EVI1 expression during primary human hematopoietic progenitor cell differentiation into various myeloid lineages. Anticancer Res. 32(11): 4883-4889

Related products

1 products available
Product options: 6

Product information

Size
Order no.
Price

Human SCF, research grade

  • E. coli
  • For research use only
10 µg
130-093-991
-

Human SCF, research grade

  • E. coli
  • For research use only
25 µg
130-096-692
-

Human SCF, premium grade

  • E. coli
  • For research use only
10 µg
130-096-693
-

Human SCF, premium grade

  • E. coli
  • For research use only
25 µg
130-096-694
-

Human SCF, premium grade

  • E. coli
  • For research use only
100 µg
130-096-695
-

Human SCF, premium grade

  • E. coli
  • For research use only
1000 µg
130-096-696
-