Human PDGF-AB

Human PDGF-AB

PDGF-AB stands for platelet derived growth factor AB. Human PDGF-AB is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human PDGF-AB can be used for a variety of applications, including:
  • Chemotaxis assays for fibroblasts, vascular smooth muscle cells, and immune cells.
  • Mesenchymal stem cell culture and differentiation.
  • Culture of hESC-derived mesenchymal stem cells .
  • Wound-healing assays and connective tissue models.

Background information

Platelet-derived growth factor AB (PDGF-AB) is a disulfide-linked heterodimer of two chains termed subunit A and B, which form different members of the PDGF family including the homodimers PDGF-AA and PDGF-BB. PDGF-AB is synthesized by megakaryocytes and stored in alpha granules in platelets, from which it is released upon stimulation with thrombin or other signals related to inflammation and injury (IL-1, IL-6, TNF-α). PDGF-AB is also produced by macrophages, endothelial cells, fibroblast, and is present in the nervous system and in muscles. By binding PDGF receptors alpha and beta (PDGFR-α and PDGFR-β), PDGF-AB acts as a potent mitogen on several mesenchymal-derived cells, including fibroblasts, smooth muscle cells, and cells of the connective tissue. PDGF-AB is not released to the circulation, but acts in the tissues in an autocrine and paracrine fashion. It is involved in hyperplasia, chemotaxis, wound healing, in the development of the nervous system, and in osteogenic differentiation. PDGFs are studied as therapeutic targets in tissue regeneration and engraftment, as well as in atherosclerosis, and fibrotic disorders and malignancies.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Biological activity

  • Proliferation of BALB/c 3T3 cells
  • research grade: ≥ 1×
    10
    6
    U/mg
Product options: 4

Product information

Size
Order no.
Price

Human PDGF-AB, research grade

  • E. coli
  • For research use only
2 µg
130-094-629
-

Human PDGF-AB, research grade

  • E. coli
  • For research use only
10 µg
130-093-979
-

Human PDGF-AB, research grade

  • E. coli
  • For research use only
100 µg
130-103-442
-

Human PDGF-AB, research grade

  • E. coli
  • For research use only
1000 µg
130-108-965
-