Human M-CSF

Human M-CSF

M-CSF stands for macrophage-colony stimulating factor. Human M-CSF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human M-CSF can be used for a variety of applications, including:
  • Survival studies and apoptosis assays, for example, using peripheral blood monocytes.
  • Differentiation of macrophages from peripheral blood monocytes.
  • Differentiation of osteoclasts from CD14+ monocytes.

Background information

Macrophage-colony stimulating factor (M-CSF), a four α-helical bundle cytokine, is a potent hematopoietic regulator. It is primarily produced by monocytes, granulocytes, endothelial cells, and fibroblasts. The main function of M-CSF is the regulation of proliferation, differentiation, and survival of monocytes, macrophages and their hematopoietic progenitors. Furthermore, M-CSF has been shown to play an important role in immunological defense, bone metabolism, fertility, and pregnancy.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of NFS-60 cells (NIBSC 89/512)
  • premium grade: ≥ 2×
    10
    7
    IU/mg
    (typical activity: ≥ 7×
    10
    7
    IU/mg
    )
  • research grade: ≥ 1×
    10
    7
    IU/mg
Product options: 6

Product information

Size
Order no.
Price

Human M-CSF, research grade

  • E. coli
  • For research use only
10 µg
130-093-963
-

Human M-CSF, research grade

  • E. coli
  • For research use only
25 µg
130-096-491
-

Human M-CSF, premium grade

  • E. coli
  • For research use only
10 µg
130-096-485
-

Human M-CSF, premium grade

  • E. coli
  • For research use only
25 µg
130-096-489
-

Human M-CSF, premium grade

  • E. coli
  • For research use only
100 µg
130-096-492
-

Human M-CSF, premium grade

  • E. coli
  • For research use only
1000 µg
130-096-493
-

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