Human M-CSF

Human M-CSF

M-CSF stands for macrophage-colony stimulating factor. Human M-CSF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human M-CSF can be used for a variety of applications, including:
  • Survival studies and apoptosis assays, for example, using peripheral blood monocytes.
  • Differentiation of macrophages from peripheral blood monocytes.
  • Differentiation of osteoclasts from CD14+ monocytes.

Background information

Macrophage-colony stimulating factor (M-CSF), a four α-helical bundle cytokine, is a potent hematopoietic regulator. It is primarily produced by monocytes, granulocytes, endothelial cells, and fibroblasts. The main function of M-CSF is the regulation of proliferation, differentiation, and survival of monocytes, macrophages and their hematopoietic progenitors. Furthermore, M-CSF has been shown to play an important role in immunological defense, bone metabolism, fertility, and pregnancy.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of NFS-60 cells (NIBSC 89/512)
  • premium grade: ≥ 2×
    10
    7
    IU/mg
    (typical activity: ≥ 7×
    10
    7
    IU/mg
    )
  • research grade: ≥ 1×
    10
    7
    IU/mg
  • Selected references

    1. Michelet, X. et al. (2015) MHC class II presentation is controlled by the lysosomal small GTPase, Arl8b. J. Immunol. 194(5): 2079-2088
    2. Vogel, S. Z. et al. (2015)
      TCAIM decreases T cell priming capacity of dendritic cells by inhibiting TLR-induced Ca
      2+
      influx and IL-2 production.
      J. Immunol. 194(7): 3136-3146
    3. Guery L. et al. (2014) Fine-tuning nucleophosmin in macrophage differentiation and activation. Blood 118: 4694-4704
    4. Meissner, F. et al. (2010) Inflammasome activation in NADPH oxidase defective mononuclear phagocytes from patients with chronic granulomatous disease. Blood 116(9): 1570-1573
    5. McEwan, W. A. et al. (2013) Intracellular antibody-bound pathogens stimulate immune signaling via the Fc receptor TRIM21. Nat. Immunol. 14(4): 327-336
    6. Bénéteau, M. et al. (2012) Combination of glycolysis inhibition with chemotherapy results in an antitumor immune response Proc. Natl. Acad. Sci. U.S.A. 109(49): 20071-20076
    7. Mire-Sluis, A.R. et al. (1995) The international standard for macrophage colony stimulating factor (M-CSF). Evaluation in an international collaborative study. J. Immunol. Methods 179: 141-151
    8. Wang, C. et al. (2010)
      Innate immune response to
      Mycobacterium tuberculosis
      Beijing and other genotypes.
      PLoS One 5(10): e13594
Product options: 6
130-093-963

Human M-CSF, research grade

E. coli
10 µg
-
130-096-491

Human M-CSF, research grade

E. coli
25 µg
-
130-096-485

Human M-CSF, premium grade

E. coli
10 µg
-
130-096-489

Human M-CSF, premium grade

E. coli
25 µg
-
130-096-492

Human M-CSF, premium grade

E. coli
100 µg
-
130-096-493

Human M-CSF, premium grade

E. coli
1000 µg
-