Human IL-2 IS

Human IL-2 IS

IL-2 IS stands for interleukin 2 "Improved Sequence", also termed aldesleukin, and is a variant of IL-2 with a serine to cysteine substitution. Human IL-2 IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays, and possesses the same properties as naturally occurring IL-2.

Applications

Human IL-2 IS can be used for a variety of applications including:
  • In vitro activation and propagation of T cells, e.g., in combination with the T Cell Activation/Expansion Kit, human.
  • In vitro stimulation of cytolytic function and expansion of NK cells, e.g., using the NK Cell Activation/Expansion Kit, human.
  • Generation of lymphokine-activated killer (LAK) cells or cytokine-induced killer (CIK) cells.

Background information

IL-2, a potent lymphoid cell growth factor, is a typical four α-helix bundle cytokine. It is produced by activated T cells, especially the CD4
+
T helper cell population. It plays an important role in both the activation and maintenance of immune responses and in lymphocyte development. IL-2 promotes proliferation and differentiation of T cells, NK cells and B cells and is involved in the elimination of self-reactive T cells. IL-2 signals through a receptor complex consisting of IL-2 receptor α-chain (CD25), β- chain, and common γ-chain. The latter two are also used for IL-15 signaling.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of CTLL-2 cells (NIBSC 86/504)
  • premium grade: ≥ 5×
    10
    6
    IU/mg
    (typical activity: ≥ 9×
    10
    6
    IU/mg
    )
  • research grade: ≥ 3×
    10
    6
    IU/mg
  • Selected References

    1. Engelmann S. et al. (2013) T cell-independent modulation of experimental autoimmune encephalomyelitis in ADAP-deficient mice. J. Immunol. 191(10): 4950-4959
    2. Nguyen, T. L. et al. (2011) Antigen-specific TGF-β-induced regulatory T cells secrete chemokines, regulate T cell trafficking, and suppress ongoing autoimmunity. J. Immunol. 187(4): 1745-1753
    3. Ng, S. B. et al. (2011) Dysregulated microRNAs affect pathways and targets of biologic relevance in nasal-type natural killer/T-cell lymphoma. Blood 118(18): 4919-4929
    4. Valignat, M. P. et al. (2013) T lymphocytes orient against the direction of fluid flow during LFA-1-mediated migration. Biophys. J. 104(2): 322-331
    5. Fuchs, S. et al. (2014)
      Patients with T
      +
      /low NK
      +
      IL-2 receptor γ chain deficiency have differentially-impaired cytokine signaling resulting in severe combined immunodeficiency.
      Eur. J. Immunol. 44(10): 3129-3140
    6. Pallett, L. J. et al. (2015) Metabolic regulation of hepatitis B immunopathology by myeloid-derived suppressor cells. Nat. Med. 21(6): 591-600
    7. Fallarini S. et al. (2012) Invariant NKT cells increase drug-induced osteosarcoma cell death. Toxicol. Sci. 167(7): 1533-1549
Product options: 6

Product information

Size
Order no.
Price

Human IL-2 IS, research grade

  • E. coli
  • For research use only
10 µg
130-097-742
-

Human IL-2 IS, research grade

  • E. coli
  • For research use only
50 µg
130-097-743
-

Human IL-2 IS, premium grade

  • E. coli
  • For research use only
10 µg
130-097-744
-

Human IL-2 IS, premium grade

  • E. coli
  • For research use only
50 µg
130-097-745
-

Human IL-2 IS, premium grade

  • E. coli
  • For research use only
200 µg
130-097-746
-

Human IL-2 IS, premium grade

  • E. coli
  • For research use only
1000 µg
130-097-748
-