Human IL-2 IS

Human IL-2 IS

IL-2 IS stands for interleukin 2 "Improved Sequence", also termed aldesleukin, and is a variant of IL-2 with a serine to cysteine substitution. Human IL-2 IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays, and possesses the same properties as naturally occurring IL-2.

Applications

Human IL-2 IS can be used for a variety of applications including:
  • In vitro activation and propagation of T cells, e.g., in combination with the T Cell Activation/Expansion Kit, human.
  • In vitro stimulation of cytolytic function and expansion of NK cells, e.g., using the NK Cell Activation/Expansion Kit, human.
  • Generation of lymphokine-activated killer (LAK) cells or cytokine-induced killer (CIK) cells.

Background information

IL-2, a potent lymphoid cell growth factor, is a typical four α-helix bundle cytokine. It is produced by activated T cells, especially the CD4
+
T helper cell population. It plays an important role in both the activation and maintenance of immune responses and in lymphocyte development. IL-2 promotes proliferation and differentiation of T cells, NK cells and B cells and is involved in the elimination of self-reactive T cells. IL-2 signals through a receptor complex consisting of IL-2 receptor α-chain (CD25), β- chain, and common γ-chain. The latter two are also used for IL-15 signaling.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of CTLL-2 cells (NIBSC 86/504)
  • premium grade: ≥ 5×
    10
    6
    IU/mg
    (typical activity: ≥ 9×
    10
    6
    IU/mg
    )
  • research grade: ≥ 3×
    10
    6
    IU/mg
Product options: 6

Product information

Size
Order no.
Price

Human IL-2 IS, research grade

  • E. coli
  • For research use only
10 µg
130-097-742
-

Human IL-2 IS, research grade

  • E. coli
  • For research use only
50 µg
130-097-743
-

Human IL-2 IS, premium grade

  • E. coli
  • For research use only
10 µg
130-097-744
-

Human IL-2 IS, premium grade

  • E. coli
  • For research use only
50 µg
130-097-745
-

Human IL-2 IS, premium grade

  • E. coli
  • For research use only
200 µg
130-097-746
-

Human IL-2 IS, premium grade

  • E. coli
  • For research use only
1000 µg
130-097-748
-

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