Human IL-1ra

Human IL-1ra

IL-1ra stands for interleukin 1 receptor antagonist. Human IL-1ra is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IL-1ra may be used for a variety of applications, including:
  • Regulation of IL-1 expression.
  • Competitive inhibition of IL-1 receptor binding ( in vitro and in vivo ).
  • Inhibition of IL-1 release.
  • Studies of anti-inflammatory effects.
  • Protection from endotoxin effects.

Alternative Names

IL1-RN

Background information

Interleukin 1 receptor antagonist (IL-1ra) is a naturally occurring anti-inflammatory protein secreted by macrophages, monocytes, neutrophils, fibroblasts, and epithelial cells. IL-1ra, a member of the IL-1 family, binds to IL-1 receptors in competition with IL-1 without inducing any cellular responses. It inhibits the activity of IL-1α and IL-1β by competitively blocking their type I and type II receptors. Furthermore, IL-1ra inhibits the secretion of IL-2 and the expression of IL-2 receptors. It has been shown that IL-1ra is capable of blocking the inflammatory responses induced by IL-1 both
in vitro
and
in vivo.
The non-glycosylated recombinant human IL-1ra expressed in
E. coli
blocks binding of IL-1 as well as the glycosylated natural form. The protein shows 26% amino acid homology to IL-1β and 19% homology to IL-1α. It has been shown that excess amounts of IL-1ra are necessary in
in vitro
systems to show significant inhibition of IL-1 induced responses.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Biological activity

  • Proliferation of mouse D10S cells
  • research grade: ≥ 2×
    10
    6
    U/mg
Product options: 1

Product information

Size
Order no.
Price

Human IL-1ra, research grade

  • E. coli
  • For research use only
20 µg
130-096-142
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