Human IL-15

Human IL-15

IL-15 stands for interleukin 15. Human IL-15 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IL-15 can be used for a variety of applications, including:
  • Activation and expansion of NK and NKT cells.
  • In vitro differentiation of NK cells, e.g., from purified CD34+ cells.
  • In vitro T cell expansion, e.g., of naive CD8+ T cells, and T cell activation, e.g., of CTLs.

Background information

IL-15 is a member of the four α-helix bundle cytokine family. It is produced by different cell types, including epithelial cells, monocytes, muscle and placenta cells. IL-15 is a potent lymphoid cell growth factor. It stimulates the proliferation of activated T cells and promotes the generation of cytotoxic T lymphocytes (CTLs). IL-15 also induces the generation, proliferation, and activation of NK cells as well as B cell growth and immunoglobulin production. In addition, IL-15 is important for the maintenance of CD8
+
memory T cells. For binding and signaling IL-15 uses the unique IL-15 receptor α-chain, but shares the β- and γ-chain of the IL-2 receptor.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of CTLL-2 cells (NIBSC 95/554)
  • premium grade: ≥ 5×
    10
    6
    U/mg
    (typical activity: ≥ 1.1×
    10
    7
    U/mg
    )
  • research grade: ≥ 2×
    10
    6
    U/mg
  • Selected references

    1. Gordy, L. E. et al. (2011) IL-15 regulates homeostasis and terminal maturation of NKT cells. J. Immunol. 187(12): 6335-6345
    2. Juelke, K. et al. (2010)
      CD62L expression identifies a unique subset of polyfunctional CD56
      dim
      NK cells.
      Blood 116(8): 1299-1307
    3. Alvarez-Breckenridge, C. A. et al. (2012) The histone deacetylase inhibitor valproic acid lessens NK cell action against oncolytic virus-infected glioblastoma cells by inhibition of STAT5/T-BET signaling and generation of gamma interferon. J. Virol. 86(8): 4566-4577
    4. Fuchs, S. et al. (2014)
      Patients with T
      +/low
      NK
      +
      IL-2 receptor γ chain deficiency have differentially-impaired cytokine signaling resulting in severe combined immunodeficiency.
      Eur. J. Immunol. 44(10): 3129-3140
    5. Dietz, L. et al. (2010) Tracking human contact allergens: from mass spectrometric identification of peptide-bound reactive small chemicals to chemical-specific naive human T-cell priming. Toxicol. Sci. 117(2): 336-347
Product options: 6
130-093-955

Human IL-15, research grade

E. coli
10 µg
-
130-095-760

Human IL-15, research grade

E. coli
25 µg
-
130-095-762

Human IL-15, premium grade

E. coli
10 µg
-
130-095-764

Human IL-15, premium grade

E. coli
25 µg
-
130-095-765

Human IL-15, premium grade

E. coli
100 µg
-
130-095-766

Human IL-15, premium grade

E. coli
1000 µg
-