Human IL-15

Human IL-15

IL-15 stands for interleukin 15. Human IL-15 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IL-15 can be used for a variety of applications, including:
  • Activation and expansion of NK and NKT cells.
  • In vitro differentiation of NK cells, e.g., from purified CD34+ cells.
  • In vitro T cell expansion, e.g., of naive CD8+ T cells, and T cell activation, e.g., of CTLs.

Background information

IL-15 is a member of the four α-helix bundle cytokine family. It is produced by different cell types, including epithelial cells, monocytes, muscle and placenta cells. IL-15 is a potent lymphoid cell growth factor. It stimulates the proliferation of activated T cells and promotes the generation of cytotoxic T lymphocytes (CTLs). IL-15 also induces the generation, proliferation, and activation of NK cells as well as B cell growth and immunoglobulin production. In addition, IL-15 is important for the maintenance of CD8
+
memory T cells. For binding and signaling IL-15 uses the unique IL-15 receptor α-chain, but shares the β- and γ-chain of the IL-2 receptor.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of CTLL-2 cells (NIBSC 95/554)
  • premium grade: ≥ 5×
    10
    6
    U/mg
    (typical activity: ≥ 1.1×
    10
    7
    U/mg
    )
  • research grade: ≥ 2×
    10
    6
    U/mg
Product options: 6

Product information

Size
Order no.
Price

Human IL-15, research grade

  • E. coli
  • For research use only
10 µg
130-093-955
-

Human IL-15, research grade

  • E. coli
  • For research use only
25 µg
130-095-760
-

Human IL-15, premium grade

  • E. coli
  • For research use only
10 µg
130-095-762
-

Human IL-15, premium grade

  • E. coli
  • For research use only
25 µg
130-095-764
-

Human IL-15, premium grade

  • E. coli
  • For research use only
100 µg
130-095-765
-

Human IL-15, premium grade

  • E. coli
  • For research use only
1000 µg
130-095-766
-

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