Human IFN-γ1b

Human IFN-γ1b

IFN-γ stands for interferon γ. Human IFN-γ is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IFN-γ1b can be used for a variety of applications, including:
  • Stimulation of monocytes, macrophages, and microglia.
  • In vitro maturation of Mo-DCs.
  • Apoptosis and cell growth inhibition assays.
  • Adhesion and migration assays.

Alternative names

IFN-γ

Background information

IFN-γ, the only type II interferon, possesses potent anti-viral activity and immunoregulatory properties. It is structurally unrelated to the type I interferons and binds to a different receptor expressed by most immune cells. IFN-γ is produced predominantly by NK cells and T cells. IFN-γ enhances NK cell and macrophage activity and regulates B cell functions, like immunglobulin production and class switching. In addition, IFN-γ orchestrates leukocyte attraction and directs growth, maturation, and differentiation of many cell types. The activity of human IFN-γ is species-specific.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of HT-29 cells (NIAID Gxg01-902-535)
  • premium grade: ≥ 2×
    10
    7
    IU/mg
    (typical activity: ≥ 5×
    10
    7
    IU/mg
    )
  • research grade: ≥ 1×
    10
    7
    IU/mg
  • Selected references

    1. Wong, V.L. et al. (1989) Growth-inhibitory activity of interferon-beta against human colorectal carcinoma cell lines. Int. J. Cancer 43: 526-530
Product options: 6
130-096-872

Human IFN-γ1b, research grade

E. coli
10 µg
-
130-096-873

Human IFN-γ1b, research grade

E. coli
25 µg
-
130-096-481

Human IFN-γ1b, premium grade

E. coli
10 µg
-
130-096-482

Human IFN-γ1b, premium grade

E. coli
25 µg
-
130-096-484

Human IFN-γ1b, premium grade

E. coli
100 µg
-
130-096-486

Human IFN-γ1b, premium grade

E. coli
1000 µg
-