Human GM-CSF

Human GM-CSF

GM-CSF stands for human granulocyte-macrophage colony–stimulating factor. Human GM-CSF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human GM-CSF can be used for a variety of applications including:
  • Cultivation of hematopoietic progenitor cells from human bone marrow in semi-solid medium.
  • In vitro generation of Mo-DCs together with Human IL-4.
  • In vitro differentiation of CD34+ cells towards eosinophils.
  • Migration assays for eosinophils.

Alternative Names

CSF2

Background information

GM-CSF is a hematopoietic growth factor, which is essential for proliferation and development of granulocyte and monocyte/macrophage progenitors. It also functions as a growth factor for erythroid and megakaryocytic precursor cells in conjunction with erythropoietin. GM-CSF is secreted by various cell types including T cells, macrophages, endothelial cells, and fibroblasts in response to inflammatory stimuli and cytokines. In addition, GM-CSF is a potent chemoattractant for neutrophils and eosinophils and enhances the effector functions of neutrophils and macrophages.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of TF-1 cells (NIBSC 88/646)
  • premium grade: ≥ 5×
    10
    6
    IU/mg
    (typical activity: ≥ 1.2×
    10
    7
    IU/mg
    )
  • research grade: ≥ 2×
    10
    6
    IU/mg
Product options: 7

Product information

Size
Order no.
Price

Human GM-CSF, research grade

  • E. coli
  • For research use only
10 µg
130-093-862
-

Human GM-CSF, research grade

  • E. coli
  • For research use only
50 µg
130-095-372
-

Human GM-CSF, premium grade

  • E. coli
  • For research use only
10 µg
130-093-864
-

Human GM-CSF, premium grade

  • E. coli
  • For research use only
50 µg
130-093-865
-

Human GM-CSF, premium grade

  • E. coli
  • For research use only
100 µg
130-093-866
-

Human GM-CSF, premium grade

  • E. coli
  • For research use only
500 µg
130-093-867
-

Human GM-CSF, premium grade

  • E. coli
  • For research use only
1000 µg
130-093-868
-

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