Human G-CSF

Human G-CSF

G-CSF stands for granulocyte colony stimulating factor. Human G-CSF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human G-CSF can be used for a variety of applications, including:
  • Growth promotion and differentiation of cells of the neutrophil lineage.
  • Induction of colony formation of normal and leukemic bone marrow cells in soft agar cultures.

Background information

Granulocyte colony stimulating factor (G-CSF) is a hematopoietic growth factor that affects proliferation and differentiation especially of progenitors of the neutrophil and granulocyte lineages. It is produced mainly by monocytes and macrophages and a variety of other cells like astrocytes, fibroblasts and endothelial cells in response to specific stimulation, for instance by endotoxin, TNF-α and IFN-γ. Furthermore, G-CSF enhances the survival and influences the immunological functions of mature neutrophils. Thus, in addition to its properties as a hematopoietic growth factor G-CSF also acts as a mediator of host defense against infection and inflammatory response.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of NFS-60 cells (NIBSC 88/502)
  • premium grade: ≥ 4×
    10
    7
    IU/mg
  • research grade: ≥ 2×
    10
    7
    IU/mg
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