Human Flt3-Ligand

Human Flt3-Ligand

Flt3-Ligand stands for Fms-related tyrosine kinase 3 ligand. Human Flt3-Ligand is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human Flt3-Ligand can be used for a variety of applications, including:
  • In vitro expansion of CD34+ hematopoietic progenitor cells.
  • Differentiation of ES-derived cells towards the hematopoietic lineage.
  • In vitro generation of Langerhans cells, dendritic cells, or eosinophils from CD34+ cells.

Background information

Fms-related tyrosin kinase 3 ligand (Flt3-Ligand) is a growth factor that regulates early hematopoiesis. Flt3-Ligand belongs to a small family of α-helical cytokines and promotes in synergy with other growth factors like G-CSF, GM-CSF, SCF, and IL-3 the proliferation and differentiation of primitive hematopoietic stem cells. Early B cell lineage differentiation as well as expansion of monocytes and immature dendritic cells is stimulated. Flt3-Ligand is expressed by T lymphocytes and bone marrow stromal fibroblasts as a membrane-bound and a soluble isoform. Both isoforms signal through the tyrosine kinase receptor Flt3/Flk-2, which is restricted to cells of hematopoietic origin. Human Flt3-Ligand is also active on mouse cells.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of OCI-AML5 cells (NIBSC 96/532)
  • premium grade: ≥ 5×
    10
    5
    U/mg
  • research grade: ≥ 2×
    10
    5
    U/mg
Product options: -

Product information

Size
Order no.
Price

Related products

1 products available