Human DKK-1

Human DKK-1

DKK-1 stands for Dickkopf-1 related protein. Human DKK-1 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human DKK-1 can be used for a variety of applications, including:
  • Cardiac differentiation of human ES and iPS cell lines.
  • Mesenchymal stem cell differentiation.
  • Studies of Wnt signaling and cell death pathways.
  • Neuronal cultures and neural differentiation of ES/iPS cells.

Background information

Dickkopf-1 related protein (DKK-1) is a member of the DKK family, which also includes DKK-2, DKK-3 and DKK-4. It was initially identified as an important embryogenic factor, especially for the development of the head. DKK-1 effects are mainly due to its role as negative regulator of Wnt signally pathway, by forming complexes with LDL-receptor-related protein 6. High expression of DKK-1 has been found during mesenchymal development, and is crucial for limbs and bones modeling, but also cardiogenesis. TNF-α plays a central role in DKK-1 induction, during arthritis, and blood levels of DKK-1 increase during multiple myeloma. DKK-1 acts also as negative regulator of neuronal survival and neural crest formation.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Biological activity

  • Proliferation of HCT116 colorectal carcinoma cells
  • research grade: ≥ 0.8×
    10
    3
    U/mg
Product options: 3

Product information

Size
Order no.
Price

Human DKK-1, research grade

  • HEK293 cells
  • For research use only
2 µg
130-103-443
-

Human DKK-1, research grade

  • HEK293 cells
  • For research use only
10 µg
130-103-444
-

Human DKK-1, research grade

  • HEK293 cells
  • For research use only
100 µg
130-103-445
-