Human BMP-4

Human BMP-4

Human BMP-4 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human BMP-4 can be used for a variety of applications, including:
  • Differentiation of human ES and iPS cells.
  • Maintenance of mouse ESCs under serum-free conditions.

Background information

Bone morphogenetic protein 4 (BMP-4) is a member of the bone morphogenetic protein family, which is part of the TGF-β superfamily. BMP-4 plays an important role in early differentiation of the embryo and in establishing of a dorsal-ventral axis. It is involved in bone and cartilage development and has been shown to play a role in the differentiation of sympathetic neurons. In synergy with Activin A it promotes the formation of definitive endoderm in human embryonic stem cells (ESCs). Furthermore, BMP-4 supports LIF (leukaemia inhibitory factor) as a positive factor for mouse ESC self-renewal and can replace the requirement of serum. The amino acid sequences of mature human BMP-4 shares 98% identity with mouse or rat BMP-4.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Biological activity

  • Induction of alkaline phosphatase assay using mouse C2C12 cells
  • premium grade: ≥ 8×
    10
    4
    U/mg
  • research grade: ≥ 4×
    10
    4
    U/mg
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