Human BMP-2

Human BMP-2

BMP-2 stands for bone morphogenetic protein 2. Human BMP-2 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human BMP-2 can be used for a variety of applications, including:
  • Study of chondrogenesis and osteogenesis.
  • Differentiation of mesenchymal stem cells into several cell type including osteoblast and cardiac cells.
  • Investigation of TGF-β signaling pathway.

Background information

Bone morphogenetic protein 2 (BMP-2) is a member of the bone morphogenetic protein family, which is part of the TGF-β superfamily. Together with BMP-7, BMP-2 is a potent stimulator of osteoblast differentiation as well as of chondrocyte proliferation of mesenchymal cells during bone and cartilage formation, and can also induce cardiac and epithelial morphogenesis. BMP-2 is secreted by several cell tissues such as spleen, lung, liver, brain, small intestine, prostate, and ovary, as immature long form, and after proteolytic cleavage it matures into an active disulfide-linked homodimer. BMP-2 signals through heterodimeric receptors, such as BMPR1A, and activates TGF-β signaling cascade and the hedgehog pathway. Besides the morphogenic role, BMP-2 is also crucial during embryonic dorsal-ventral generation and organogenesis. Recombinant BMP-2 is under clinical investigation for treatment of bone degeneration diseases, spinal injuries, and orthodontic pathologies. Mature human mouse and rat BMP-2 share full amino acid sequence identity. Recombinant Human BMP-2 corresponds to the mature form of BMP-2.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Biological activity

  • Alkaline phosphatase assay using mouse C2C12 fibroblasts (NIBSC 93/574)
  • premium grade: ≥ 1×
    10
    5
    U/mg
  • research grade: ≥ 5×
    10
    4
    U/mg
  • Selected references

    1. Cheng, H. et al. (2003) Osteogenic activity of the fourteen types of human bone morphogenetic proteins (BMPs). J Bone Joint Surg Am 85(A8): 1544-1552
Product options: 6
130-110-923

Human BMP-2, research grade

E. coli
10 µg
-
130-110-922

Human BMP-2, research grade

E. coli
25 µg
-
130-110-924

Human BMP-2, premium grade

E. coli
10 µg
-
130-110-925

Human BMP-2, premium grade

E. coli
25 µg
-
130-110-926

Human BMP-2, premium grade

E. coli
100 µg
-
130-110-927

Human BMP-2, premium grade

E. coli
1000 µg
-