CytoCapture, hexagonal (250-100)

CytoCapture, hexagonal (250-100)

Cytocapture H250-100 products allow clustering of groups of non adherent or adherent cells to facilitate experiments where a controlled clustering and analysis of cells is required. The product is also available with low cell adhesion (LCA) surface. They may be used for applications like:
  • Clustering up to 100 adherent suspension cells per microcavity
  • Controlled formation and investigation of cellular spheroids in LCA product
  • Sensitive fluorescence analysis (FRET, FRAP, FLIM)
  • Cell signaling studies, cell-cell Interaction and communication
  • Time lapse video microscopy
  • Individual cell characterization and spheroid picking
The CytoCapture H250-100 and H250-100, LCA products are designed for cell based assays and high resolution life cell imaging of cell cluster and cell spheroids or co-culture analysis. A uniform array of hexagonal polymer cavities lays on top of the cover glass bottoms of these specialized cell culture and imaging consumables. Individual cavities have a distance from surrounding wall to wall of 250 µm. The wall thickness Is 10 µm. Height of the walls Is 100 µm. The walls of the cavities errect straight upright from the bottom (F-shape). Seeded cells sediment spontaneously inside the microcavities. The microcavities are ether hydrophilic (130-098-298) or coated with a Low Cell Adhesion (LCA) surface (130-098-299). The geometry of the cavities allows groups of cells to enter. On the hydrophilic surface the cells are kept in colonies. On the LCA surface cell adhesion to the surfaces is suppressed and the cells form spontaneously spheroids. Within these cavities the spheroids are protected from general fluid convection within the cell culture consumable. The microcavities allow that the cell clusters or spheroids can be tracked over days without major change of their position. The microcavities allow also a better size control and homogenous size distribution of the spheroids.
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