CliniMACS® Cytokine Capture System (IFN-gamma) – Product Line

CliniMACS
®
Cytokine Capture System (IFN-gamma) – Product Line

Interferon-gamma (IFN-gamma) is a cytokine secreted by CD4
+
and CD8
+
memory and effector T cells upon antigenic restimulation.
The CliniMACS® Cytokine Capture System (IFN-gamma) – Product Line is comprised of the CliniMACS IFN-gamma Catchmatrix Reagent, consisting of CD45 antibodies conjugated to IFN-gamma specific antibodies, and the CliniMACS IFN-gamma Enrichment Reagent.
The CliniMACS IFN-gamma Enrichment Reagent consists of murine anti- IFN-gamma monoclonal antibodies conjugated to superparamagnetic iron dextran particles. The CliniMACS IFN- gamma Enrichment Reagent allows the magnetic selection of IFN-gamma–secreting cells.
One kit is suitable for processing up to 1 × 10
9
total cells.
Please inquire about required CliniMACS System components and accessories.

Disclaimer

The CliniMACS
®
System components, including Reagents, Tubing Sets, Instruments, and PBS/EDTA Buffer, are designed, manufactured and tested under a quality system certified to ISO 13485.
In the EU, the CliniMACS System components are available as CE-marked medical devices for their respective intended use, unless otherwise stated. The CliniMACS Reagents and Biotin Conjugates are intended for in vitro use only and are not designated for therapeutic use or direct infusion into patients. The CliniMACS Reagents in combination with the CliniMACS System are intended to separate human cells. Miltenyi Biotec as the manufacturer of the CliniMACS System does not give any recommendations regarding the use of separated cells for therapeutic purposes and does not make any claims regarding a clinical benefit. For the manufacturing and use of target cells in humans the national legislation and regulations - e.g., for the EU the Directive 2004/23/EC ("human tissues and cells"), or the Directive 2002/98/EC ("human blood and blood components") - must be followed. Thus, any clinical application of the target cells is exclusively within the responsibility of the user of a CliniMACS System.
In the US, the CliniMACS CD34 Reagent System, including the CliniMACS Plus Instrument, CliniMACS CD34 Reagent, CliniMACS Tubing Sets TS and LS, and the CliniMACS PBS/EDTA Buffer, is FDA approved; all other products of the CliniMACS Product Line are available for use only under an approved Investigational New Drug (IND) application or Investigational Device Exemption (IDE).
CliniMACS MicroBeads are for research use only and not for human therapeutic or diagnostic use. Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research only and not for therapeutic or diagnostic use.

Applications

The CliniMACS Cytokine Capture System (IFN-gamma) – Product Line allows for the concomitant enrichment of viable antigen-specific CD4
+
and CD8
+
IFN- gamma–secreting T cells.

Referenced literature

Antigen-specific CD4 and CD8 T cells can be enriched using the CliniMACS Cytokine Capture System (IFN-gamma)
1
.
A successful enrichment of T cells with the specificity for HCMV
2
, AdV
3
, EBV
4
, and
Aspergillus5
, as well as leukemia-reactive T cells
6
has been demonstrated.
Adoptively transferred antigen-specific T cells have been shown to restore protective immunity and control established AdV
7
, HCMV
8
, and EBV
9
infections after hematopoietic stem cell transplantations.
Video

Automatic isolation of antigen-specific T cells for adoptive T cell therapy

CD4+ and CD8+ T cells stimulated in vitro with a specific antigen can be magnetically labeled with the CliniMACS® Cytokine Capture System (IFN-gamma). In combination with the new CliniMACS Prodigy, the system allows fast and fully automated generation of antigen-specific T cells in a closed sterile system.

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Product information

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CliniMACS Cytokine Capture System (IFN-gamma) (279-01)

  • CE
200-070-111
-