CD95 antibodies

CD95 antibodies

Clone: DX2
DX2 recognizes human CD95, also known as FAS and Apo-1. It is a member of the tumor necrosis factor receptor superfamily (TNFR) and is found on the surface of many normal and neoplastically transformed cells. Its ligand, CD95L (FASL/Apo-1L), is able to induce apoptosis in CD95-expressing cells upon binding. CD95 and CD95L are up-regulated on lymphocytes upon activation and are known to play a key role in the regulation of an inflammatory response: Juxtocrine “fratricide” of neighbouring lymphocytes via mutual CD95 and CD95L expression helps to terminate immune responses, while apoptosis of pro-inflammatory cells via CD95 helps maintain immune privilege in sites such as the eye, where CD95L is found to be expressed in the retina and cornea. Cross-linking of CD95 receptors by DX2 monoclonal antibody has been described to induce apoptosis in certain target cells.

Applications

Reagent can be used for immunophenotyping by flow cytometry. Abnormal numbers of cells expressing this antigen or aberrant expression levels of the antigen can be expected in some disease states. It is important to understand the normal expression pattern for this antigen and its relationship to expression of other relevant antigens in order to perform appropriate analysis.
Expression of CD95 may be used as aid to diagnostic in the characterization of samples from individuals suspected with hematologic neoplasia.

Technical specifications

  • Antigen: CD95
  • Clone: DX2
  • Isotype: mouse IgG1κ
  • Molecular mass of antigen [kDa]: 35
  • Distribution of antigen: B cells, epithelial cells, fibroblasts, heart, kidney, liver, lung, monocytes, neutrophils, ovary, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC
  • Selected References

    1. Pitcher, C. J. et al. (2002)
      Development and homeostasis of T cell memory in
      rhesus macaque
      .
      J. Immunol. 168: 29-43
    2. Griffith, T. et al. (1995) Fas ligand-induced apoptosis as a mechanism of immune privilege. Science 270: 1189-1192
    3. Stelzer GT et al. (1997) U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry 30(5): 214-230
    4. Lynch, D. et al. (1995) Fas and FasL in the homeostatic regulation of immune responses. Immunol. Today 16: 569-574
    5. Rothe, G et al. (2012) Consensus protocol for the flow cytometric Immunophenotyping of hematopoietic malignancies. Leukemia 10(5): 877-895
    6. van Dongen, J. J. M. et al. (2012) EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 26(9): 1908-1975
    7. Clinical and Laboratory Standards Institute (CLSI) (2007) Clinical Flow Cytometric Analysis of Neoplastic Hematolympoid Cells, CLSI document H43-A2 (ISBN 1-56238-635-2) CLSI; Approved Guideline - Second Edition
    8. Clinical and Laboratory Standards Institute (CLSI) (2007) Enumeration of Immunologically Defined Cell Populations by Flow Cytometry, CLSI document H42-A2 (ISBN 1-56238-640-9) CLSI; Approved Guideline - Second Edition
    9. Kishimoto, T et al. (eds) Leucocyte typing VI: White cell differentiation antigens. Garland Publishing Inc., New York
  • Brochures and posters

  • Certificates of Analysis (CoA)

    Please follow this
    link
    to search for certificates by lot number.
Product options: 2
170-078-058

CD95-PE

for 100
tests
-
170-078-086

CD95-APC

for 100
tests
-