CD62L antibodies

CD62L antibodies

Clone: 145/15
145/15 recognizes the human CD62L antigen, a 74 kDa glycoprotein and a member of the selectin family of cell surface molecules. CD62L is also known as L-selectin, LECAM-1, or LAM-1 and binds a series of glycoproteins including CD34, GlyCAM-1, and MAdCAM-1. CD62L is important for homing of naive lymphocytes via endothelial venules to peripheral lymph nodes and Peyer’s patches. The CD62L antigen also contributes to the recruitment of leukocytes from the blood to areas of inflammation. Most hematopoietic cells express CD62L, including many peripheral blood B cells, T cells, monocytes, granulocytes, and some myeloid cells from bone marrow, and thymocytes. CD62L is continuously endoproteolytically cleaved from the cell surface neutrophils and lymphocytes (shedding). Proteolysis is accelerated, for example, after antigenic activation of T cells.
Always use fresh material for immunofluorescent staining of CD62L
+
cells. For optimal results, the cells should not be older than 8–12 hours. Keep cells continuously cold. CD62L-expression may be rapidly lost due to shedding.

Applications

Reagent can be used for immunophenotyping by flow cytometry. Abnormal numbers of cells expressing this antigen or aberrant expression levels of the antigen can be expected in some disease states. It is important to understand the normal expression pattern for this antigen and its relationship to expression of other relevant antigens in order to perform appropriate analysis.
Expression of CD62L may be used as aid to diagnostic in the characterization of samples from individuals suspected with hematologic neoplasia.

Technical specifications

  • Antigen: CD62L
  • Clone: 145/15
  • Isotype: mouse IgG2a
  • Molecular mass of antigen [kDa]: 38
  • Distribution of antigen: B cells, bone marrow, eosinophils, granulocytes, Langerhans cells, lymphocytes, monocytes, neutrophils, NK cells, red blood cells, spleen, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, VioGreen, PE-Vio770
  • Certificates of Analysis (CoA)

    Please follow this
    link
    to search for certificates by lot number.
Product options: 3

Product information

Size
Order no.
Price

CD62L-FITC

  • 145/15
  • CE–IVD
for 100
tests
170-078-034
-

CD62L-VioGreen

  • 145/15
  • CE–IVD
for 100
tests
170-080-025
-

CD62L-PE-Vio770

  • 145/15
  • CE–IVD
for 100
tests
170-080-026
-