CD27 antibodies

CD27 antibodies

Clone: M-T271
M-T271 recognizes CD27, a member of the tumor necrosis factor receptor family. CD27 is expressed at differing levels on memory B cells, a fraction of plasma cells, and on naive and memory T cells, but is not expressed on naive B cells or effector T cells. It is also expressed on NK cells. CD27 therefore represents a useful marker to distinguish certain T and B cell subsets from each other.

Applications

Reagent can be used for immunophenotyping by flow cytometry. Abnormal numbers of cells expressing this antigen or aberrant expression levels of the antigen can be expected in some disease states. It is important to understand the normal expression pattern for this antigen and its relationship to expression of other relevant antigens in order to perform appropriate analysis.
Expression of CD27 may be used as aid to diagnostic in the characterization of samples from individuals suspected with hematologic neoplasia.

Alternative Names

S152, TNFRSF7

Technical specifications

  • Antigen: CD27
  • Clone: M-T271
  • Isotype: mouse IgG1κ
  • Alternative names of antigen: S152, TNFRSF7
  • Molecular mass of antigen [kDa]: 27
  • Distribution of antigen: B cells, NK cells, plasma cells, red blood cells, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, APC, VioBlue
  • Selected References

    1. Stelzer GT et al. (1997) U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry 30(5): 214-230
    2. Rothe, G et al. (2012) Consensus protocol for the flow cytometric Immunophenotyping of hematopoietic malignancies. Leukemia 10(5): 877-895
    3. van Dongen, J. J. M. et al. (2012) EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 26(9): 1908-1975
    4. Schlossman, S.F. et al. (1993) Leukocyte Typing V. Oxford, Oxford University Press
    5. Clinical and Laboratory Standards Institute (CLSI) (2007) Clinical Flow Cytometric Analysis of Neoplastic Hematolympoid Cells, CLSI document H43-A2 (ISBN 1-56238-635-2) CLSI; Approved Guideline - Second Edition
    6. Clinical and Laboratory Standards Institute (CLSI) (2007) Enumeration of Immunologically Defined Cell Populations by Flow Cytometry, CLSI document H42-A2 (ISBN 1-56238-640-9) CLSI; Approved Guideline - Second Edition
  • Brochures and posters

  • Certificates of Analysis (CoA)

    Please follow this
    link
    to search for certificates by lot number.
Product options: 3
170-078-023

CD27-FITC

for 100
tests
-
170-078-024

CD27-APC

for 100
tests
-
170-078-064

CD27-VioBlue

for 100
tests
-