CD117 antibodies

CD117 antibodies

Clone: A3C6E2
A3C6E2 recognizes human CD117, also known as c-kit or SCF receptor. It is a 145 kDa receptor tyrosine kinase that is involved in stem cell signaling, activation, and proliferation. The CD117 antigen is expressed on about 1–3% of mononuclear cells from peripheral blood mononuclear cells (PBMCs) or cord blood, and on up to 10% of bone marrow cells. CD117 is further expressed on basophils, myeloid dendritic cells, TCRα/β
+
T cells, CD19
+
B cells, and CD56
+
NK cells, as well as on mast cells, melanocytes, and AML (acute myeloid leukemia) blasts.

Applications

Reagent can be used for immunophenotyping by flow cytometry. Abnormal numbers of cells expressing this antigen or aberrant expression levels of the antigen can be expected in some disease states. It is important to understand the normal expression pattern for this antigen and its relationship to expression of other relevant antigens in order to perform appropriate analysis.
Expression of CD117 may be used as aid to diagnostic in the characterization of samples from individuals suspected with hematologic neoplasia.

Alternative Names

c-kit, PBT, SCFR, KIT

Technical specifications

  • Antigen: CD117
  • Clone: A3C6E2
  • Isotype: mouse IgG1κ
  • Alternative names of antigen: c-kit, PBT, SCFR, KIT
  • Molecular mass of antigen [kDa]: 107
  • Distribution of antigen: B cells, bone marrow, brain, cancer cells, endothelial cells, fibroblasts, hematopoietic stem cells, kidney, leukemia cells, liver, lung, mast cells, megakaryocytes, myeloid cells, NK cells, red blood cells, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC
  • Selected references

    1. Kubo, H. et al. (2008) Increased cardiac myocyte progenitors in failing human hearts. Circulation 118: 649-657
    2. Wood BL et al. (2007) 2006 Bethesda International Consensus recommendations on the immunophenotypic analysis of hematolymphoid neoplasia by flow cytometry: optimal reagents and reporting for the flow cytometric diagnosis of hematopoietic neoplasia. Cytometry B Clin. Cytom. 72: 14-22
    3. Stelzer GT et al. (1997) U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry 30(5): 214-230
    4. Rothe, G et al. (2012) Consensus protocol for the flow cytometric Immunophenotyping of hematopoietic malignancies. Leukemia 10(5): 877-895
    5. van Dongen, J. J. M. et al. (2012) EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 26(9): 1908-1975
    6. Bühring, H. J. et al. (1991) The product of the proto-oncogene c-kit (P145c-kit) is a human bone marrow surface antigen of hematopoietic precursor cells which is expressed on a subset of acute non-lymphoblastic cells. Leukemia 5: 854-860
    7. Clinical and Laboratory Standards Institute (CLSI) (2007) Clinical Flow Cytometric Analysis of Neoplastic Hematolympoid Cells, CLSI document H43-A2 (ISBN 1-56238-635-2) CLSI; Approved Guideline - Second Edition
    8. Clinical and Laboratory Standards Institute (CLSI) (2007) Enumeration of Immunologically Defined Cell Populations by Flow Cytometry, CLSI document H42-A2 (ISBN 1-56238-640-9) CLSI; Approved Guideline - Second Edition
    9. Kishimoto, T et al. (eds) Leucocyte typing VI: White cell differentiation antigens. Garland Publishing Inc., New York
  • Brochures and posters

  • Certificates of Analysis (CoA)

    Please follow this
    link
    to search for certificates by lot number.
Product options: 2
170-078-037

CD117-PE

for 100
tests
-
170-078-038

CD117-APC

for 100
tests
-