Anti-TCRα/β antibodies

Anti-TCRα/β antibodies

Clone: BW242/412
BW242/412 recognizes the human α/β T cell receptor (TCR). The T cell receptor (TCR) is a heterodimer composed of two transmembrane glycoprotein chains, α and β. Both chains are members of the Ig superfamily and consist of a constant and a polymorphic variable region. The variable region of the TCRα/β is involved in recognition of antigenic peptides presented by the MHC complex of antigen-presenting cells. The Anti-TCRα/β antibody, BW242/412, recognizes a common determinant of the TCRα/β-CD3 complex.

Applications

Reagent can be used for immunophenotyping by flow cytometry. Abnormal numbers of cells expressing this antigen or aberrant expression levels of the antigen can be expected in some disease states. It is important to understand the normal expression pattern for this antigen and its relationship to expression of other relevant antigens in order to perform appropriate analysis. Expression of TCRα/β may be used as aid to diagnostic in the characterization of samples from individuals suspected with hematologic neoplasia.

Technical specifications

  • Antigen: TCRα/β
  • Clone: BW242/412
  • Isotype: mouse IgG2b
  • Distribution of antigen: T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: APC
  • Selected references

    1. Spencer, C. T. et al. (2008) Only a subset of phosphoantigen-responsive gamma9delta2 T cells mediate protective tuberculosis immunity. J. Immunol. 181(7): 4471-4484
    2. Voogt, P. J. et al. (1989)
      Normal hematopoietic progenitor cells and malignant lymphohematopoietic cells show different susceptibility to direct cell-mediated MHC-non-restricted lysis by T cell receptor
      /CD3
      , T cell receptor gamma delta
      +
      /CD3
      +
      and T cell receptor-alpha beta
      +
      /CD3
      +
      lymphocytes.
      J. Immunol. 142(5): 1774-1780
    3. Borst, J. et al. (1988) Distinct molecular forms of human T cell receptor gamma/delta detected on viable T cells by a monoclonal antibody. J. Exp. Med. 167(5): 1625-1644
    4. Lanier, L. L. et al. (1987)
      The T cell antigen receptor complex expressed on normal peripheral blood CD4
      , CD8
      T lymphocytes. A CD3-associated disulfide-linked gamma chain heterodimer.
      J. Exp. Med. 165(4): 1076-1094
    5. Conrad, M. L. et al. (2007) TCR and CD3 antibody cross-reactivity in 44 species. Cytometry A 71(11): 925-933
    6. Lanier, L. L. et al. (1988) Structural and serological heterogeneity of γ/δ T cell antigen receptor expression in thymus and peripheral blood. Eur. J. Immunol. 18(12): 1985-1992
    7. Testi, R. and Lanier, L. L. (1989) Functional expression of CD28 on T cell antigen receptor gamma/delta-bearing T lymphocytes. Eur. J. Immunol. 19(1): 185-188
    8. Lanier, L. L. et al. (1987) The gamma T-cell antigen receptor. J. Clin. Immunol. 7(6): 429-440
    9. Moens, E. et al. (2011) IL-23R and TCR signaling drives the generation of neonatal Vγ9 Vδ2 T cells expressing high levels of cytotoxic mediators and producing IFN-γ and IL-17. J. Leukoc. Biol. 89(5): 743-752
    10. Wood BL et al. (2007) 2006 Bethesda International Consensus recommendations on the immunophenotypic analysis of hematolymphoid neoplasia by flow cytometry: optimal reagents and reporting for the flow cytometric diagnosis of hematopoietic neoplasia. Cytometry B Clin. Cytom. 72: 14-22
    11. Stelzer GT et al. (1997) U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry 30(5): 214-230
    12. Rothe, G et al. (2012) Consensus protocol for the flow cytometric Immunophenotyping of hematopoietic malignancies. Leukemia 10(5): 877-895
    13. van Dongen, J. J. M. et al. (2012) EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 26(9): 1908-1975
    14. Clinical and Laboratory Standards Institute (CLSI) (2007) Clinical Flow Cytometric Analysis of Neoplastic Hematolympoid Cells, CLSI document H43-A2 (ISBN 1-56238-635-2) CLSI; Approved Guideline - Second Edition
    15. Clinical and Laboratory Standards Institute (CLSI) (2007) Enumeration of immunologically defined cell populations by flow cytometry, CLSI document H42-A2 (ISBN 1-56238-640-9) CLSI; Approved Guideline - Second Edition
  • Brochures and posters

  • Certificates

    Please follow this
    link
    to download the Certificate of Conformity (CoC) by lot number.
Product options: 2
170-078-046

Anti-TCRα/β-PE

for 100
tests
-
170-078-047

Anti-TCRα/β-APC

for 100
tests
-