MACS® ART Annexin V principle

How does MACS ART Annexin V work?

  • Selective removal of apoptotic spermatozoa from sperm preparations
  • Increased quality of purified sperm populations
  • Find the fittest for improved subsequent in vitro fertilization

How does it work?

The depletion of apoptotic sperm cells begins with magnetic labeling of apoptotic sperm cells by the MACS ART Annexin V Reagent. The labeled sperm cells are then passed through a separation column located in a magnetic field. Unwanted sperm cells are selectively retained in the column. Non-apoptotic spermatozoa are not labeled by the reagent, so they pass through the column and are collected for any IVF application.

MACS ART Annexin V Protocol

Depletion of apoptotic spermatozoa using the MACS ART Annexin V System is simple and fast. It adapts easily into laboratories to enhance classical sperm parameters and lower sperm apoptotic markers. Additionally, it can be combined with other sperm preparation techniques for procedural flexibility.

References

  • Ziarati N, et al. Hum Fertil (Camb). 2018 Jan; 24:1-8
  • Esbert A, et al. Andrology. 2017 Jul; 5(4):807-813
  • Berteli, T.S, et al. Andrology. 2017 Jul; 5(4):776-782
  • Sánchez-Martín, P et al. Reprod Biomed Online. 2017 May; 34(5):506-512
  • Romany L, et al. J Assist Reprod Genet. 2017 Feb; 34(2):201-207
  • Troya J, et al. JBRA Assisted Reproduction. 2015; 19(2):66-69
  • Degheidy T, et al. Andrologia. 2015 Oct; 47(8):892-6 
  • Lukaszuk K et al, AJP Rep. 2015 Oct; 5(2): e105–e108
  • Gil M, et al. J Assist Reprod Genet. 2013 Apr;30(4):479-85
  • Herrero MB, et al. J Assist Reprod Genet. 2013 Nov;30(11):1415-9
  • Rawe V Y, et al. Reprod Biomed Online. 2010 Mar;20(3):320-3
  • Dirican E K, et al. J Assist Reprod Genet. 2008 Aug;25(8):375-81
  • Aziz N, et al. Human Reproduction 2007 22:1413-1419
  • Grunewald S, et al. Cell and tissue Banking 2006 7:99-104
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