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Why are recombinant antibodies important in flow cytometry?

Researchers and opinion leaders have considered a number of potential solutions to the so-called antibody-related reproducibility “crisis”. One is to transition from traditional monoclonal antibodies to recombinant antibodies*. 

So, what makes recombinant antibodies better and more effective than conventional hybridoma-derived antibodies?

We asked six experts from the flow cytometry and antibody community to share their opinion and experience with recombinant antibodies.  

Six experts. Six perspectivesOne opinion about recombinant antibodies.

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“..approximately 50 % of all antibodies, that are sold, appear not to work.”

Andrew Bradbury, Ph.D. Chief Scientific Officer, Specifica Inc., United States

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“…the whole field is suffering from poor specificity of antibodies.”

Karl Johan Malmberg, M.D., Ph.D., Professor of Immunology, Oslo University Hospital, Norway

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“It´s crucially important for us, that we have high-quality reagents to minimize variation between experiments.”

James Hutchinson, MB BChir, Ph.D. Department of Surgery, University Hospital Regensburg, Germany

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“We have seen that there is a huge advantage in terms of the background signal. ”

Anis Larbi, Ph.D., Head Flow Cytometry Platform, Principal Investigator Immunity and AgingA*STAR Institute, Singapore

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“ I strongly believe that in the future, it will really pay off tolook at recombinant material.”

Dr. Susanne Heck, Head of Flow Cytometry Research Platform, BRC, Kings College London, United Kingdom

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“…this is an important gain to improve panel precision and to limit the variability of results being produced.”

Joffrey de la Larichaudy, Ph.D., Deputy Director of QC, CELLforCURE, France