Simple and fast enrichment of cross presenting DCs

Cross-presenting conventional dendritic cells (DCs) play a powerful role in the induction of protective cytotoxic T lymphocyte (CTL) response against cancer and infections. Cross-presenting DCs specifically express the XCR1 receptor, however, they are difficult to isolate due to their very low frequency.

Thanks to the combination of our recombinant REAfinity™ Anti-XCR1 mouse antibodies with MACS®Technology and optimal sample preparation products, XCR1+ cross presenting DCs can now be routinely enriched in just 35 minutes with good recovery and purity without the need for time-consuming pre-depletion of non-target cells or laborious flow sorting.

Before separation
Before separation
After separation
After separation

XCR1+ dendritic cells (DCs) were isolated from spleen single-cell suspension using Anti-XCR1 MicroBead Kit (Spleen) with two MS Columns and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-MHC Class II-Vio® Green, CD11c-VioBlu® , Anti-XCR1-PE and CD8a-APC and analyzed using the MACSQuant® Analyzer 10.
Cell debris, dead cells, and autofluorescent cells were excluded from analysis based on scatters and propidium iodide fluorescence. Dot plots on the right show conventional DCs gated on CD11c+ MHC class I+ cells, stained for cross-presenting DC markers (CD8a and XCR1).


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