Free application note

Microchip-based cell sorting for maximum cell viability

In this application note, we demonstrate the gentle sorting character of the MACSQuant® Tyto® by sequentially sorting highly viable lymphocyte populations of B cells, T cells, and natural killer (NK) cells from only one original sample of peripheral blood mononuclear cells (PBMCs).

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The collection of viable cells is paramount to successful cell sorting via flow cytometry. It is well known that high pressures, decompression, and shear forces negatively affect cell viability and functionality during conventional droplet sorting.

The MACSQuant Tyto provides a novel approach to cell sorting, eliminating the above-mentioned concerns:

  • Nozzle-free: Unlike on conventional droplet sorters, cells are sorted by a high-speed mechanical valve within the MACSQuant Tyto cartridge
  • Low pressure: Cells are sorted in a constant liquid stream of very low pressure (3 psi), preventing shear forces
  • No droplets: In the closed cartridge, cells do not get decompressed or charged and no potentially hazardous droplets form
  • Temperature controlled: The sorting chamber can be temperature controlled allowing constant cooling of the sample throughout the whole sorting procedure

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