Novel astrocyte-specific monoclonal antibodies

Specific antibodies are essential for reliable neural cell studies, especially for cell types with high diversity, such as astrocytes. GFAP, the most commonly used astrocyte marker, is a reliable marker for reactive astrocytes. However, non-reactive astrocytes located in healthy CNS tissue or remote from CNS lesions are difficult to be detected by GFAP immunohistochemical staining1.

Unlike GFAP, GLAST (EAAT1, Slc1a3) is found on virtually all astrocytes, including reactive and non-reactive astrocytes, throughout the brain, e.g., in the cerebellum, cortex, and hippocampus. After successful generation of the first monoclonal anti-GLAST antibody2 by Miltenyi Biotec, a second astrocyte-specific antibody was also developed by immunizing rats with astrocytes isolated from GFAP-EGFP transgenic mice: The Anti-ACSA-2 (anti-astrocyte cell surface antigen-2) antibody.

Immunocytochemical, immunohistochemical, and flow cytometric analyses show that the ACSA-2 antigen is specifically expressed on GLAST-positive astrocytes and that the Anti-ACSA-2 antibody is highly specific for all astrocyte lineage cells, both neonatal and adult3 (fig. 1). Recently the ubiquitously expressed astrocyte protein ATP1B2 was identified as ACSA-2 epitope and the Anti-ACSA-2 antibody was recommended as the “first-choice method for astrocyte isolation and characterization”4


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Immunocytochemical and immunohistochemical analysis of ACSA-2 and GLAST co-expression.

Interested? Try our unique astrocyte-specific antibodies and benefit from:

  • Monoclonal astrocyte-specific antibody recognizing astrocyte cell membrane antigen
  • Pan marker for both, non-reactive and reactive astrocytes
  • Tested for immunocytochemistry, immunohistochemistry, and flow cytometry analysis

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References:

  1. Sofroniew, M.V. et al. (2010) Astrocytes: biology and pathology. Acta Neuropathologica 119(1): 7–35. (PMID: 20012068)
  2. Jungblut, M. et al. (2012) Isolation and characterization of living primary astroglial cells using the new GLAST-specific monoclonal antibody ACSA-1. Glia 60(6): 894-907. (PMID: 22374709)
  3. Kantzer, C.G. et al. (2017) Anti-ACSA-2 defines a novel monoclonal antibody for prospective isolation of living neonatal and adult astrocytes. Glia 65(6): 990-1004. (PMID: 28317180)
  4. Batiuk, M.Y. et al. (2017) An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody. J Biol Chem 292(21): 8874-8891. (PMID: 28373281)