General T cells

A complete workflow for all your T cell needs

  • Isolation of virtually any T cell subset
  • Cultivation, activation, expansion, and polarization of T cells
  • Multiparameter flow analysis of T cells

Application data by workflow step

T cell isolation

Magnetic T cell isolation with MACS® Technology

Reliability now comes with better performance and faster protocols. Our improved MicroBeads and T Cell Isolation Kits allow for high-quality separation of  human and mouse T cells within a few minutes. 

Comparison of activation marker expression of mouse CD4+ and CD8+ T cells isolated with MACS MicroBeads or T Cell Isolation Kits
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MACS MicroBeads

  • Updated CD4 and CD8a MicroBeads, mouse allow for positive selection of T cells in only 13.5 minutes

    T cell isolation kits

  • Untouched isolation of human and mouse T cells and T cell subsets in only 18 minutes

T cell isolation directly from blood products

StraightFrom™ MicroBeads allow magnetic cell separation directly from human blood products, such as whole blood, buffy coat, or leukoreduction system chambers (LRSCs). CD4+ and CD8+ T cell subsets can be purified using the autoMACS® Pro Separator, the MultiMACS™ Cell24 Separator Plus, or manual separators.


Comparison of StraightFrom Buffy Coat MicroBeads with other commercially available products
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StraightFrom MicroBeads

  • No density gradient centrifugation, no erythrocyte lysis

  • Easy and fast protocols with only a few handling steps

  • No cell activation and no effects on cell biology



Label-free T cell isolation

REAlease™ Technology allows for magnetic cell isolation with subsequent removal of any beads and labels from your cells of interest. Download the scientific poster below to get the whole story!

REAlease MicroBeads

  • Bead-free cells suited for a second positive selection
  • Label-free cells for any downstream application
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Scientific poster
REAlease Immunomagnetic Separation Technology with reversible labeling for postive selection of leukocytes

Philipp Steinbrueck, Zhong Yu, Ursula Bissa, Jennifer Brieden, and Christian Dose, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany

Isolation of cytokine-producing T cells

Cytokine Secretion Assay – Cell Enrichment and Detection Kits are designed for magnetic enrichment and highly sensitive detection of cytokine-secreting cells. This unique technology enables detection of one antigen-specific T cell per million cells. Isolated cells can be immediately used for downstream applications, e.g. cell culture and flow cytometric analysis.

Principle of the MACS Cytokine Secretion Assays
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Cytokine Secretion Assay – Cell Enrichment and Detection Kits

  • Unique technology for the isolation of viable cytokine-secreting cells

  • Accurate analysis of cytokine secretion at single-cell level

  • Sensitive detection of one T cell per million cells

  • Available for human and mouse cells secreting TNF-a, GMCSF, IFN-a, IFN-g, or IL-2, -4, -5, -10, -12, -13, -17, -22

T cell culture

T cell maintenance

TexMACS Medium is an optimized, serum-free cell culture medium developed for reliable T cell and Treg cell culture results. Chemically-defined and without animal-derived components, it allows for the highest reproducibility in human and mouse cell culture applications.

Comparison of expansion rates of human CD4+ and CD8+ T cells in TexMACS Medium or competitor’s T cell medium using the T Cell Activation/Expansion Kit, human
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TexMACS Medium

  • Free of serum and other animal-derived components

  • Reliable T cell activation and high expansion rates

  • Up to MACS GMP grade

  • Available with or without phenol red

T cell activation and expansion

We offer a variety of polyclonal and antigen-specific T cell stimulation tools for you to choose from. Find your different options below:

 Expansion of human and mouse CD4+ and CD8+ T cells using T Cell Activation/Expansion Kits
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T cell Activation/Expansion Kits 

  • MACSiBead™ Particles loaded with functional-grade antibodies

  • Efficient activation and expansion of human, mouse, and non-human primate T cells or Treg cells.

Activation and expansion of CD4+ and CD8+ T cells with T Cell TransAct
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T Cell TransAct™ Stimulation Reagent

  • Ready-to-use reagent for activation and expansion of human T cells

  • Simple volumetric dosage and easy removal by washing

  • Up to MACS GMP grade

Comparison of T cell activation using the CytoStim Reagent, SEB, or PMA/Ionomycin
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CytoStim™ Reagent

  • Non-toxic alternative to the superantigen SEB for the stimulation of human T cells

Production of IFN-γ by human CD4+ or CD8+ T cells after stimulation with different antigens using PepTivator Peptide Pools
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PepTivator® Peptide Pools

  • Stimulation of both CD4+ and CD8+ T cells with our extensive panel of antigens

  • 15-mer peptides with 11-amino acid overlap that cover the complete sequence of the respective antigen

  • Available in 96-well format

  • MACS GMP-grade peptide pools for translational settings

MACS Handbook:

Cell culture

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T cell polarization

We provide a broad selection of superior-quality cytokines with high biological activity at a competitive price. Three different grades, research, premium, and MACS GMP grade, provide flexibility in your assay setup.

Human IL-2 IS biological activity
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MACS Premium-Grade Cytokines 

  • Well-defined biological activities, normalized to international reference standards (IU/mg)

  • Exact unit dosing for reproducible results

  • No requirement for laborious pre-testing

Mouse TH cell polarization using CytoBoxes (TH 1, TH 2, or TH 17) in combination with either RPMI or TexMACS Medium
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TH cell polarization with CytoBoxes 

  • Convenient all-in-one starter kits for polarization of mouse TH1, TH2 and TH17 cells

  • Recombinant cytokines and functional-grade blocking antibodies

Multiparameter flow analysis of T cells

As the final step in many workflows, flow analysis plays an important part in various T cell applications. The following flow reagents can help you make sure that the data you generate on your flow cytometer are reliable and consistent:

  • REAfinity Recombinant Antibodies recombinantly generated antibodies that ensure lot-to-lot consistency and eliminate background signal
  • MACSplex Cytokine Kits analysis of multiple soluble cytokines in one sample using any standard flow cytometer
  • Rapid Cytokine Inspector – combined surface marker and intracellular cytokine staining for multiparameter analysis of activated human T cells
  • Cytokine Secretion Assay – Detection Kits – flow analysis of cytokine secretion at a detection level of one T cell in a million
  • Build your own T cell flow panel with our Flow Panel Builder
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Application note
Flow cytometry analysis of TH subsets

Find out more about multiparameter flow analysis of your favorite TH cell subsets.

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