Differentiation of pluripotent stem cells

Reproducible differentiation of pluripotent stem cells (PSCs)

  • Reproducible, feeder-free culture up to MACS® GMP-grade
  • Rapid enrichment of lineage-specific cells
  • Multicolor phenotypic flow analysis

Application protocols

Discover our different pluripotent stem cell workflows and find the one that fits your experimental needs.

Application data by workflow step

Pluripotent stem cell culture

Efficient and reproducible cell culture results are key for the successful differentiation of human pluripotent stem cells (PSCs) in disease modelling, drug screening, or cell therapy research. Our range of xeno-free media, high-quality growth factors, and small molecules provides you with reliable solutions for robust and reproducible stem cell culture.

StemMACS™ iPS-Brew XF Medium

When differentiating your pluripotent stem cells, you want to be sure that your cells are highly pluripotent and can readily differentiate into any downstream lineage. StemMACS iPS-Brew XF is a cell culture medium for PSCs that gives you full flexibility with your downstream differentiation protocols, e.g., cardiomyocyte or neural differentiation.

Cardiomyocytes differentiated from human PSCs,  analyzed by flow cytometry and immunofluorescence microscopy
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Expansion and maintenance of PSCs
StemMACS iPS-Brew XF is a xeno-free media formulation for the maintenance and expansion of PSCs under feeder-free conditions. It enables robust expansion over multiple passages, while cells maintain a pluripotent phenotype. PSCs can be differentiated into various cell types that display the desired phenotype, including for example cardiomyocytes and neural cells.


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Application note
Performance of StemMACS iPS-Brew XF in the maintenance and neural differentiation of human pluripotent stem cells

Xun Cheng and Gavin Wang, Stem Cell Core, Department of Genetics, Stanford University, Stanford, CA, 94305, USA

Human FGF-2 IS activity assay
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Reproducible differentiation results with exact unit dosing of cytokines
MACS® Premium-Grade Cytokines come with the specific biological activity for each lot. This enables reproducible unit dosing and eliminates the need to test each new cytokine lot. Dosing cytokines in units removes one variable from your differentiation experiments and thereby increases reproducibility.

Enrichment of PSCs

PSCs can differentiate into virtually any cell type in vitro. MACS MicroBead Technology allows for rapid isolation of cells from specific lineages at any time point during differentiation and can be used to deplete unwanted cells or enrich differentiated cells of interest.

Differentiation of PSCs into hepatocyte-like cells by selection of CXCR4 (CD184)+ definitive endoderm (DE) cells
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Pure lineage-specific cells with MACS MicroBeads
Differentiation of PSCs into pure cell populations of interest can be greatly enhanced by magnetically isolating lineage-specific cells during the differentiation period. MACS MicroBead Technology provides a broad range of marker-specific cell enrichment solutions, such as the isolation of hepatocyte-like cells using the lineage-specific marker CXCR4.

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Scientific poster
Highly efficient differentiation of hPSC into hepatocyte-like cells by selection of CXCR4 (CD184)+ definitive endoderm (DE) cells

Annett Kurtz, Andreas Bosio, and Sebastian Knoebe, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany


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