Microglia

Isolation and characterization of microglia

  • Gentle and automated dissociation of brain tissue
  • Viable single-cell suspensions free of myelin debris
  • Isolation of pure and viable microglia from neonatal and adult mouse, rat, and human tissue

Application protocols

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Video
How to isolate pure viable microglia

Watch this hands-on protocol showing you how to isolate microglial cells from adult mouse brain. The Miltenyi Biotec protocol is faster, standardized, reproducible, and easy to scale up.

Application data by workflow step

Dissociation of murine brain tissue

Purity and viability of cells before and after sample clearing

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Viable and pure adult brain cells

Dissociate adult brain tissue into pure single-cell suspensions containing a minimum amount of cell debris and erythrocytes, ready for downstream isolation and analysis of microglia.

Purity of cell suspensions obtained from murine (P4) whole brain tissue

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Functional neonatal neural cells

Benefit from preserved surface epitopes for enhanced microglia-specific antibody binding due to gentle combined enzymatic digestion and mechanical dissociation.

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Webinar
MACS Academy: Adult brain dissociation and neural cell isolation

Join Dr. Hui Zhang and her webinar on the latest options and integrated solutions for neuroscience research and the use of MACS Technology for adult brain dissociation and neural cell isolation.

Microglia isolation and flow cytometry analysis

Our MACS® MicroBead Technology, in combination with the Adult Brain Dissociation Kit provides a gentle technique with minimal manipulation time. It allows you to obtain pure, viable and functional adult microglia.

video preview Play video

Video
How to isolate pure viable microglia

Watch this hands-on protocol showing you how to isolate microglia from adult mouse brain in a fast, standardized, reproducible, and easy-to-scale-up way.

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Scientific poster
Standardized tissue dissociation and removal of myelin debris optimize isolation of cells from human brain tumor

Sandy Reiß¹, Stephan Dützmann², Sandra Pennartz¹

¹Miltenyi Biotec GmbH,  Bergisch Gladbach, Germany, ²Department of Neurosurgery, Johann Wolfgang Goethe University, Frankfurt am Main , Germany

Immunostaining and microscopy analysis of microglia

CD68 staining of adult mouse microglia

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Staining of microglia-specific marker CD68

Stain and analyze cultured microglial cells or PFA-fixed cryosections of mouse brain with pure  CD68 antibody.

CD11b staining of adult mouse microglia

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CD11b staining of adult mouse microglia

Microglia in culture or cryosections of adult hippocampus or olfactory bulb can be visualized by immunocytochemistry or immunohistochemistry staining with the pure CD11b antibody.

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