Monocytes and MoDCs

Isolation of monocytes and generation of  monocyte derived dendritic cells

  • Fast and easy isolation of CD14+ monocytes
  • Generation of Mo-DCs
  • Translating Mo-DC research for clinical purposes

Application protocols

Discover our different workflows for monocytes and Mo-DCs and find the one that fits your experimental needs.

Application data by workflow step

Isolation of classical and non-classical monocytes

Increasing number of studies focus on the distinct functions of classical an non-classical monocyte subsets. We have developed specific kits for the separation of monocytes and monocyte subsets.

Flow cytometry analysis of enriched CD14+ cells.
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Highly pure CD14+ monocytes

CD14 MicroBeads enable the isolation of viable CD14monocytes to high purities, with high yields. The isolated monocytes are suited for subsequent differentiation into Mo-DCs.

CD14+ monocytes isolation directly from blood products

Using our new StraightFrom™ Portfolio we provide tools for fast and easy isolation of monocytes directly from whole blood, buffy coat, and leukapheresis without the need for density gradient centrifugation,  wash steps, erythrocyte lysis, and cell counting. Using StraightFrom™ Buffy Coat CD14 MicroBead Kit, human you can get pure CD14+ monocytes directly from buffy coat in less than 30 minutes.

Analysis gate
CD14+ cells

Isolation of CD14+ monocytes from buffy coat. Separation of a buffy coat sample using the StraightFrom™ Buffy Coat CD14 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns. Cells were fluorescently stained with CD15-APC, CD14-PE, as well as CD45‑VioBlue® and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Generation of Mo-DCs 

Large numbers of DCs for basic research and immunotherapies can be generated in vitro from monocytes using several differentiation/maturation protocols. To obtain functional Mo-DCs that activate T cells efficiently, reliable procedures using high-quality reagents are necessary.

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Scientific Poster
Monocyte-derived dendritic cells (Mo-DCs) generated with CliniMACS Prodigy® are functional and fulfill requirements for cancer vaccines

Mareke Brüning, Tobias Ozimkowski, Eva Bergschneider, Michael Birth, Stefan Wild, and Andrzej Dzionek 

Miltenyi Biotec GmbH, Bergisch Gladbach, Germany 

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Webinar
MACS Academy: Translational Mo-DC research

Join Cristina Conforti Andreoni and her webinar on the past, present, and future of monocyte-derived dendritic cells in translational research. She covers the challenges and solutions of Mo-DC manufacturing for clinical research and the means to bringing DC therapy to the next level.

Related PDFs:

Generation of Mo-DCs (application note)

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