Neuronal cells were isolated from P2 and from 9 weeks old CD-1 ® mouse brains. P2 mouse brain was dissociated using the Neural Tissue Dissociation Kit – Postnatal Neurons and adult mouse brain using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, and the microglia-specific antibody CD11b-FITC. Cells were analyzed by flow cytometry using the MACSQuant™ Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
Before separation | Isolated neuronal cells | Non-neuronal cells |
  View details Figure 1 Neuronal cells were isolated from P2 and from 9 weeks old CD-1 ® mouse brains. P2 mouse brain was dissociated using the Neural Tissue Dissociation Kit – Postnatal Neurons and adult mouse brain using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, and the microglia-specific antibody CD11b-FITC. Cells were analyzed by flow cytometry using the MACSQuant™ Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |  View details Figure 1 Neuronal cells were isolated from P2 and from 9 weeks old CD-1 ® mouse brains. P2 mouse brain was dissociated using the Neural Tissue Dissociation Kit – Postnatal Neurons and adult mouse brain using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, and the microglia-specific antibody CD11b-FITC. Cells were analyzed by flow cytometry using the MACSQuant™ Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |  View details Figure 1 Neuronal cells were isolated from P2 and from 9 weeks old CD-1 ® mouse brains. P2 mouse brain was dissociated using the Neural Tissue Dissociation Kit – Postnatal Neurons and adult mouse brain using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, and the microglia-specific antibody CD11b-FITC. Cells were analyzed by flow cytometry using the MACSQuant™ Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
 View details Figure 1 Neuronal cells were isolated from P2 and from 9 weeks old CD-1 ® mouse brains. P2 mouse brain was dissociated using the Neural Tissue Dissociation Kit – Postnatal Neurons and adult mouse brain using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, and the microglia-specific antibody CD11b-FITC. Cells were analyzed by flow cytometry using the MACSQuant™ Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |  View details Figure 1 Neuronal cells were isolated from P2 and from 9 weeks old CD-1 ® mouse brains. P2 mouse brain was dissociated using the Neural Tissue Dissociation Kit – Postnatal Neurons and adult mouse brain using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, and the microglia-specific antibody CD11b-FITC. Cells were analyzed by flow cytometry using the MACSQuant™ Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |  View details Figure 1 Neuronal cells were isolated from P2 and from 9 weeks old CD-1 ® mouse brains. P2 mouse brain was dissociated using the Neural Tissue Dissociation Kit – Postnatal Neurons and adult mouse brain using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, and the microglia-specific antibody CD11b-FITC. Cells were analyzed by flow cytometry using the MACSQuant™ Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
 View details Figure 1 Neuronal cells were isolated from P2 and from 9 weeks old CD-1 ® mouse brains. P2 mouse brain was dissociated using the Neural Tissue Dissociation Kit – Postnatal Neurons and adult mouse brain using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, and the microglia-specific antibody CD11b-FITC. Cells were analyzed by flow cytometry using the MACSQuant™ Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |  View details Figure 1 Neuronal cells were isolated from P2 and from 9 weeks old CD-1 ® mouse brains. P2 mouse brain was dissociated using the Neural Tissue Dissociation Kit – Postnatal Neurons and adult mouse brain using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, and the microglia-specific antibody CD11b-FITC. Cells were analyzed by flow cytometry using the MACSQuant™ Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |  View details Figure 1 Neuronal cells were isolated from P2 and from 9 weeks old CD-1 ® mouse brains. P2 mouse brain was dissociated using the Neural Tissue Dissociation Kit – Postnatal Neurons and adult mouse brain using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, and the microglia-specific antibody CD11b-FITC. Cells were analyzed by flow cytometry using the MACSQuant™ Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
Adult cells |
Before separation | Isolated neuronal cells | Non-neuronal cells |
 View details Figure 1 Neuronal cells were isolated from P2 and from 9 weeks old CD-1 ® mouse brains. P2 mouse brain was dissociated using the Neural Tissue Dissociation Kit – Postnatal Neurons and adult mouse brain using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, and the microglia-specific antibody CD11b-FITC. Cells were analyzed by flow cytometry using the MACSQuant™ Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |  View details Figure 1 Neuronal cells were isolated from P2 and from 9 weeks old CD-1 ® mouse brains. P2 mouse brain was dissociated using the Neural Tissue Dissociation Kit – Postnatal Neurons and adult mouse brain using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, and the microglia-specific antibody CD11b-FITC. Cells were analyzed by flow cytometry using the MACSQuant™ Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |  View details Figure 1 Neuronal cells were isolated from P2 and from 9 weeks old CD-1 ® mouse brains. P2 mouse brain was dissociated using the Neural Tissue Dissociation Kit – Postnatal Neurons and adult mouse brain using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, and the microglia-specific antibody CD11b-FITC. Cells were analyzed by flow cytometry using the MACSQuant™ Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
 View details Figure 1 Neuronal cells were isolated from P2 and from 9 weeks old CD-1 ® mouse brains. P2 mouse brain was dissociated using the Neural Tissue Dissociation Kit – Postnatal Neurons and adult mouse brain using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, and the microglia-specific antibody CD11b-FITC. Cells were analyzed by flow cytometry using the MACSQuant™ Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |  View details Figure 1 Neuronal cells were isolated from P2 and from 9 weeks old CD-1 ® mouse brains. P2 mouse brain was dissociated using the Neural Tissue Dissociation Kit – Postnatal Neurons and adult mouse brain using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, and the microglia-specific antibody CD11b-FITC. Cells were analyzed by flow cytometry using the MACSQuant™ Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |  View details Figure 1 Neuronal cells were isolated from P2 and from 9 weeks old CD-1 ® mouse brains. P2 mouse brain was dissociated using the Neural Tissue Dissociation Kit – Postnatal Neurons and adult mouse brain using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, and the microglia-specific antibody CD11b-FITC. Cells were analyzed by flow cytometry using the MACSQuant™ Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
 View details Figure 1 Neuronal cells were isolated from P2 and from 9 weeks old CD-1 ® mouse brains. P2 mouse brain was dissociated using the Neural Tissue Dissociation Kit – Postnatal Neurons and adult mouse brain using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, and the microglia-specific antibody CD11b-FITC. Cells were analyzed by flow cytometry using the MACSQuant™ Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |  View details Figure 1 Neuronal cells were isolated from P2 and from 9 weeks old CD-1 ® mouse brains. P2 mouse brain was dissociated using the Neural Tissue Dissociation Kit – Postnatal Neurons and adult mouse brain using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, and the microglia-specific antibody CD11b-FITC. Cells were analyzed by flow cytometry using the MACSQuant™ Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |  View details Figure 1 Neuronal cells were isolated from P2 and from 9 weeks old CD-1 ® mouse brains. P2 mouse brain was dissociated using the Neural Tissue Dissociation Kit – Postnatal Neurons and adult mouse brain using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator. Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, and the microglia-specific antibody CD11b-FITC. Cells were analyzed by flow cytometry using the MACSQuant™ Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
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