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REAlease Technology allows for magnetic cell isolation and subsequent removal of any beads and labels from the cells in just a few simple steps. The technology relies on recombinantly engineered antibody fragments instead of whole antibodies for cell surface labeling. Monomeric fragments have a low affinity for cell surface markers. However, when multimerized as part of the REAlease Complex, the fragments bind markers with high avidity. REAlease MicroBeads bound to the REAlease Complex facilitate cell isolation using MACS® Columns and Separators. Disruption of the complex by adding REAlease Release Reagent after cell isolation leads to monomerization and spontaneous dissociation of the antibody fragments from the cell surface, resulting in label-free cells.