CD309 (Flk-1) MicroBead Kit, mouse

CD309 (Flk-1) MicroBead Kit, mouse

The CD309 (Flk-1) MicroBead Kit has been developed for the isolation of CD309 (Flk-1)
+
cells from differentiated murine ES or iPS cell cultures. Isolation of CD309
+
cells from heterogeneous differentiation cultures can improve differentiation into specific hematopoietic or cardiovascular lineages, e.g. endothelial cells.

Background information

CD309 is a class 3 receptor tyrosine kinase, also known as Flk-1 (fetal liver kinase 1), vascular endothelial growth factor receptor 2 (VEGFR2), or kinase insert domain receptor (KDR). CD309 is one of the earliest differentiation markers for endothelial and hematopoietic cells.
1,2
Using mouse embryonic stem cells it has been shown that CD309 is a marker for multipotent cardiovascular progenitor cells that give rise to cardiomyocyte, endothelial, and vascular smooth muscle cell lineages.
3,4
Note
: For optimal flow cytometric analysis of isolated cells after separation we recommend Labeling Check Reagent.

Columns

LS or autoMACS
®
Columns.
  • Selected references

    1. Shalaby, F. et al. (1995) Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice. Nature 376: 62-66
    2. Eichmann, A. et al. (1997) Ligand-dependent development of the endothelial and hemopoietic lineages from embryonic Proc. Natl. Acad. Sci. U.S.A. 94: 5141-5146
    3. Kattmann et al. (2006) Dev. Cell 5: 723-732
    4. Yamashita, J. et al. (2000) Flk1-positive cells derived from embryonic stem cells serve as vascular progenitors. Nature 408(6808): 92-96
Product options: 1
for 1×
10
9
total cells
-

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