Name: IL-6 Antibody, anti-mouse
Availability: InStock

Data and images
Specifications

Product data and images for IL-6 Antibody, anti-mouse

Figures

Figure 1

Peritoneal cells were isolated from a BALB/c mouse injected with thioglycollate five days before. Adherent cells were stimulated with LPS for six hours. After two hours, brefeldin A was added. The cells were harvested and intracellularly stained with Anti-IL-6 and with CD115-APC or -PE. IL-6 staining controls (left images) were done by incubating cells with 100 μg/mL of Anti-IL-6 pure – functional grade antibody, prior to staining. Cells were analyzed by flow cytometry using the MACSQuant

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Analyzer. Gating was performed according to forward scatter and side scatter properties of the cells.

Anti-IL-6-PE staining control	Stimulated sample
View details Figure 1 Peritoneal cells were isolated from a BALB/c mouse injected with thioglycollate five days before. Adherent cells were stimulated with LPS for six hours. After two hours, brefeldin A was added. The cells were harvested and intracellularly stained with Anti-IL-6 and with CD115-APC or -PE. IL-6 staining controls (left images) were done by incubating cells with 100 μg/mL of Anti-IL-6 pure – functional grade antibody, prior to staining. Cells were analyzed by flow cytometry using the MACSQuant ^® Analyzer. Gating was performed according to forward scatter and side scatter properties of the cells.	View details Figure 1 Peritoneal cells were isolated from a BALB/c mouse injected with thioglycollate five days before. Adherent cells were stimulated with LPS for six hours. After two hours, brefeldin A was added. The cells were harvested and intracellularly stained with Anti-IL-6 and with CD115-APC or -PE. IL-6 staining controls (left images) were done by incubating cells with 100 μg/mL of Anti-IL-6 pure – functional grade antibody, prior to staining. Cells were analyzed by flow cytometry using the MACSQuant ^® Analyzer. Gating was performed according to forward scatter and side scatter properties of the cells.
Anti-IL-6-APC staining control	Stimulated sample
View details Figure 1 Peritoneal cells were isolated from a BALB/c mouse injected with thioglycollate five days before. Adherent cells were stimulated with LPS for six hours. After two hours, brefeldin A was added. The cells were harvested and intracellularly stained with Anti-IL-6 and with CD115-APC or -PE. IL-6 staining controls (left images) were done by incubating cells with 100 μg/mL of Anti-IL-6 pure – functional grade antibody, prior to staining. Cells were analyzed by flow cytometry using the MACSQuant ^® Analyzer. Gating was performed according to forward scatter and side scatter properties of the cells.	View details Figure 1 Peritoneal cells were isolated from a BALB/c mouse injected with thioglycollate five days before. Adherent cells were stimulated with LPS for six hours. After two hours, brefeldin A was added. The cells were harvested and intracellularly stained with Anti-IL-6 and with CD115-APC or -PE. IL-6 staining controls (left images) were done by incubating cells with 100 μg/mL of Anti-IL-6 pure – functional grade antibody, prior to staining. Cells were analyzed by flow cytometry using the MACSQuant ^® Analyzer. Gating was performed according to forward scatter and side scatter properties of the cells.

Specifications for IL-6 Antibody, anti-mouse

Overview

Interleukin 6 (IL-6), originally identified as a B cell differentiation factor, is a multifunctional cytokine, which regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions. It induces the terminal maturation of activated B cells into antibody-secreting plasma cells and acts in synergy with IL-3 to support the proliferation of hematopoietic stem cells. IL-6 is produced by many cell types, such as monocytes, fibroblasts, endothelial cells, eosinophils, and T cells. Disturbed IL-6 production has been associated with pathological processes, including inflammatory autoimmune diseases and cancer.

Detailed product information

Technical specifications

Clone	MP5-20F3
Clonality	monoclonal
Isotype	rat IgG1κ, rat IgG1
Isotype control	Isotype Control Antibody, rat IgG1
Host	rat
Type of antibody	Primary antibodies, Functional-grade antibodies
Species	mouse
Antigen	IL-6
Molecular mass of antigen [kDa]	22
Entrez Gene ID	16193
RRID	AB_2660386, AB_2660387, AB_2660379

Data and images

Data and images for IL-6 Antibody, anti-mouse

Figures

Figure 1

^®

Analyzer. Gating was performed according to forward scatter and side scatter properties of the cells.

Anti-IL-6-PE staining control	Stimulated sample
View details Figure 1 Peritoneal cells were isolated from a BALB/c mouse injected with thioglycollate five days before. Adherent cells were stimulated with LPS for six hours. After two hours, brefeldin A was added. The cells were harvested and intracellularly stained with Anti-IL-6 and with CD115-APC or -PE. IL-6 staining controls (left images) were done by incubating cells with 100 μg/mL of Anti-IL-6 pure – functional grade antibody, prior to staining. Cells were analyzed by flow cytometry using the MACSQuant ^® Analyzer. Gating was performed according to forward scatter and side scatter properties of the cells.	View details Figure 1 Peritoneal cells were isolated from a BALB/c mouse injected with thioglycollate five days before. Adherent cells were stimulated with LPS for six hours. After two hours, brefeldin A was added. The cells were harvested and intracellularly stained with Anti-IL-6 and with CD115-APC or -PE. IL-6 staining controls (left images) were done by incubating cells with 100 μg/mL of Anti-IL-6 pure – functional grade antibody, prior to staining. Cells were analyzed by flow cytometry using the MACSQuant ^® Analyzer. Gating was performed according to forward scatter and side scatter properties of the cells.
Anti-IL-6-APC staining control	Stimulated sample
View details Figure 1 Peritoneal cells were isolated from a BALB/c mouse injected with thioglycollate five days before. Adherent cells were stimulated with LPS for six hours. After two hours, brefeldin A was added. The cells were harvested and intracellularly stained with Anti-IL-6 and with CD115-APC or -PE. IL-6 staining controls (left images) were done by incubating cells with 100 μg/mL of Anti-IL-6 pure – functional grade antibody, prior to staining. Cells were analyzed by flow cytometry using the MACSQuant ^® Analyzer. Gating was performed according to forward scatter and side scatter properties of the cells.	View details Figure 1 Peritoneal cells were isolated from a BALB/c mouse injected with thioglycollate five days before. Adherent cells were stimulated with LPS for six hours. After two hours, brefeldin A was added. The cells were harvested and intracellularly stained with Anti-IL-6 and with CD115-APC or -PE. IL-6 staining controls (left images) were done by incubating cells with 100 μg/mL of Anti-IL-6 pure – functional grade antibody, prior to staining. Cells were analyzed by flow cytometry using the MACSQuant ^® Analyzer. Gating was performed according to forward scatter and side scatter properties of the cells.

Specifications

Specifications for IL-6 Antibody, anti-mouse

Overview

Detailed product information

Technical specifications

Clone	MP5-20F3
Clonality	monoclonal
Isotype	rat IgG1κ, rat IgG1
Isotype control	Isotype Control Antibody, rat IgG1
Host	rat
Type of antibody	Primary antibodies, Functional-grade antibodies
Species	mouse
Antigen	IL-6
Molecular mass of antigen [kDa]	22
Entrez Gene ID	16193
RRID	AB_2660386, AB_2660387, AB_2660379

IL-6 Antibody, anti-mouse

IL-6 Antibody, anti-mouse

Product data and images for IL-6 Antibody, anti-mouse

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Specifications for IL-6 Antibody, anti-mouse

Overview

Detailed product information

Technical specifications

Data and images

Data and images for IL-6 Antibody, anti-mouse

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Specifications

Specifications for IL-6 Antibody, anti-mouse

Overview

Detailed product information

Technical specifications

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IL-6 Antibody, anti-mouse

IL-6 Antibody, anti-mouse

Product data and images for IL-6 Antibody, anti-mouse

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Specifications for IL-6 Antibody, anti-mouse

Overview

Detailed product information

Technical specifications

Data and images

Data and images for IL-6 Antibody, anti-mouse

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Specifications

Specifications for IL-6 Antibody, anti-mouse

Overview

Detailed product information

Technical specifications

Related products for IL-6 Antibody, anti-mouse

2 products available

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IL-6 Antibody, anti-mouse