Name: IL-21 Antibody, anti-human, REAfinity™
Availability: InStock

Data and images
Specifications

Product data and images for IL-21 Antibody, anti-human, REAfinity™

Figures

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 µg/mL ionomycin for 6 hours. After two hours 1 µg/mL brefeldin A was added. Cells were then fixed, permeabilized, and stained with Anti-IL-21 antibodies as well as with CD154 and CD4 antibodies. CD4

⁺

cells were pre-gated for the analysis. Flow cytometry was performed using the MACSQuant

^®

Analyzer. Cell debris were excluded from the analysis based on scatter signals.

View details Figure 1 Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 µg/mL ionomycin for 6 hours. After two hours 1 µg/mL brefeldin A was added. Cells were then fixed, permeabilized, and stained with Anti-IL-21 antibodies as well as with CD154 and CD4 antibodies. CD4 ⁺ cells were pre-gated for the analysis. Flow cytometry was performed using the MACSQuant ^® Analyzer. Cell debris were excluded from the analysis based on scatter signals.	View details Figure 1 Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 µg/mL ionomycin for 6 hours. After two hours 1 µg/mL brefeldin A was added. Cells were then fixed, permeabilized, and stained with Anti-IL-21 antibodies as well as with CD154 and CD4 antibodies. CD4 ⁺ cells were pre-gated for the analysis. Flow cytometry was performed using the MACSQuant ^® Analyzer. Cell debris were excluded from the analysis based on scatter signals.
View details Figure 1 Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 µg/mL ionomycin for 6 hours. After two hours 1 µg/mL brefeldin A was added. Cells were then fixed, permeabilized, and stained with Anti-IL-21 antibodies as well as with CD154 and CD4 antibodies. CD4 ⁺ cells were pre-gated for the analysis. Flow cytometry was performed using the MACSQuant ^® Analyzer. Cell debris were excluded from the analysis based on scatter signals.	View details Figure 1 Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 µg/mL ionomycin for 6 hours. After two hours 1 µg/mL brefeldin A was added. Cells were then fixed, permeabilized, and stained with Anti-IL-21 antibodies as well as with CD154 and CD4 antibodies. CD4 ⁺ cells were pre-gated for the analysis. Flow cytometry was performed using the MACSQuant ^® Analyzer. Cell debris were excluded from the analysis based on scatter signals.
View details Figure 1 Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 µg/mL ionomycin for 6 hours. After two hours 1 µg/mL brefeldin A was added. Cells were then fixed, permeabilized, and stained with Anti-IL-21 antibodies as well as with CD154 and CD4 antibodies. CD4 ⁺ cells were pre-gated for the analysis. Flow cytometry was performed using the MACSQuant ^® Analyzer. Cell debris were excluded from the analysis based on scatter signals.	View details Figure 1 Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 µg/mL ionomycin for 6 hours. After two hours 1 µg/mL brefeldin A was added. Cells were then fixed, permeabilized, and stained with Anti-IL-21 antibodies as well as with CD154 and CD4 antibodies. CD4 ⁺ cells were pre-gated for the analysis. Flow cytometry was performed using the MACSQuant ^® Analyzer. Cell debris were excluded from the analysis based on scatter signals.

Specifications for IL-21 Antibody, anti-human, REAfinity™

Overview

Clone REA1039 recognizes the human interleukin 21 (IL-21), which is secreted by a certain subset of CD4

⁺

T cells, called T follicular helper (Tfh) cells, also characterized by the expression of CXCR5 and ICOS and the lineage-specific transcription factor BCL6. But IL-21 production can also be found in other T helper cell lineages and natural killer T (NKT) cells. IL-21 acts in a autocrine manner on Tfh cells and is critical for antibody production by B cells, it enhances natural killer (NK) cell functions, and promotes proliferation of CD8

⁺

T cell.

Additional information: Clone REA1039 displays negligible binding to Fc receptors.

Alternative names

CVID11, Za11

Detailed product information

Technical specifications

Clone	REA1039
Clonality	monoclonal
Isotype	recombinant human IgG1
Isotype control	REA Control Antibody (I), human IgG1
Host	cell line
Type of antibody	Primary antibodies, Recombinant antibodies
Species	human
Antigen	IL-21
Alternative names of antigen	CVID11, Za11
Molecular mass of antigen [kDa]	15
Distribution of antigen	T cells, CD4+ T cells, T helper cells, Tfh cells
Entrez Gene ID	59067
RRID	AB_2727992, AB_2727941, AB_2727993, AB_2727942, AB_2727994, AB_2727943

Data and images

Data and images for IL-21 Antibody, anti-human, REAfinity™

Figures

Figure 1

⁺

cells were pre-gated for the analysis. Flow cytometry was performed using the MACSQuant

^®

Analyzer. Cell debris were excluded from the analysis based on scatter signals.

View details Figure 1 Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 µg/mL ionomycin for 6 hours. After two hours 1 µg/mL brefeldin A was added. Cells were then fixed, permeabilized, and stained with Anti-IL-21 antibodies as well as with CD154 and CD4 antibodies. CD4 ⁺ cells were pre-gated for the analysis. Flow cytometry was performed using the MACSQuant ^® Analyzer. Cell debris were excluded from the analysis based on scatter signals.	View details Figure 1 Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 µg/mL ionomycin for 6 hours. After two hours 1 µg/mL brefeldin A was added. Cells were then fixed, permeabilized, and stained with Anti-IL-21 antibodies as well as with CD154 and CD4 antibodies. CD4 ⁺ cells were pre-gated for the analysis. Flow cytometry was performed using the MACSQuant ^® Analyzer. Cell debris were excluded from the analysis based on scatter signals.
View details Figure 1 Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 µg/mL ionomycin for 6 hours. After two hours 1 µg/mL brefeldin A was added. Cells were then fixed, permeabilized, and stained with Anti-IL-21 antibodies as well as with CD154 and CD4 antibodies. CD4 ⁺ cells were pre-gated for the analysis. Flow cytometry was performed using the MACSQuant ^® Analyzer. Cell debris were excluded from the analysis based on scatter signals.	View details Figure 1 Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 µg/mL ionomycin for 6 hours. After two hours 1 µg/mL brefeldin A was added. Cells were then fixed, permeabilized, and stained with Anti-IL-21 antibodies as well as with CD154 and CD4 antibodies. CD4 ⁺ cells were pre-gated for the analysis. Flow cytometry was performed using the MACSQuant ^® Analyzer. Cell debris were excluded from the analysis based on scatter signals.
View details Figure 1 Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 µg/mL ionomycin for 6 hours. After two hours 1 µg/mL brefeldin A was added. Cells were then fixed, permeabilized, and stained with Anti-IL-21 antibodies as well as with CD154 and CD4 antibodies. CD4 ⁺ cells were pre-gated for the analysis. Flow cytometry was performed using the MACSQuant ^® Analyzer. Cell debris were excluded from the analysis based on scatter signals.	View details Figure 1 Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 µg/mL ionomycin for 6 hours. After two hours 1 µg/mL brefeldin A was added. Cells were then fixed, permeabilized, and stained with Anti-IL-21 antibodies as well as with CD154 and CD4 antibodies. CD4 ⁺ cells were pre-gated for the analysis. Flow cytometry was performed using the MACSQuant ^® Analyzer. Cell debris were excluded from the analysis based on scatter signals.

Specifications

Specifications for IL-21 Antibody, anti-human, REAfinity™

Overview

Clone REA1039 recognizes the human interleukin 21 (IL-21), which is secreted by a certain subset of CD4

⁺

T cell.

Additional information: Clone REA1039 displays negligible binding to Fc receptors.

Alternative names

CVID11, Za11

Detailed product information

Technical specifications

Clone	REA1039
Clonality	monoclonal
Isotype	recombinant human IgG1
Isotype control	REA Control Antibody (I), human IgG1
Host	cell line
Type of antibody	Primary antibodies, Recombinant antibodies
Species	human
Antigen	IL-21
Alternative names of antigen	CVID11, Za11
Molecular mass of antigen [kDa]	15
Distribution of antigen	T cells, CD4+ T cells, T helper cells, Tfh cells
Entrez Gene ID	59067
RRID	AB_2727992, AB_2727941, AB_2727993, AB_2727942, AB_2727994, AB_2727943

IL-21 Antibody, anti-human, REAfinity™

IL-21 Antibody, anti-human, REAfinity™

Product data and images for IL-21 Antibody, anti-human, REAfinity™

Figures

Figure 1

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Specifications for IL-21 Antibody, anti-human, REAfinity™

Overview

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Detailed product information

Technical specifications

Data and images

Data and images for IL-21 Antibody, anti-human, REAfinity™

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Specifications

Specifications for IL-21 Antibody, anti-human, REAfinity™

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IL-21 Antibody, anti-human, REAfinity™

IL-21 Antibody, anti-human, REAfinity™

Product data and images for IL-21 Antibody, anti-human, REAfinity™

Figures

Figure 1

Figure 1

Figure 1

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Figure 1

Figure 1

Specifications for IL-21 Antibody, anti-human, REAfinity™

Overview

Alternative names

Detailed product information

Technical specifications

Data and images

Data and images for IL-21 Antibody, anti-human, REAfinity™

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Specifications

Specifications for IL-21 Antibody, anti-human, REAfinity™

Overview

Alternative names

Detailed product information

Technical specifications

Related products for IL-21 Antibody, anti-human, REAfinity™

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