Recombinant human IL-12 (interleukin 12) can promote proliferation of NK and T cells, regulates their cytotoxic activity, and can induce Th1 differentiation of T cells. The T cell-stimulating factor IL-12 is a crucial regulator of cell mediated immune responses as it also induces the production of IFN-γ by PBMCs, among other cytokines. The recombinant protein is manufactured for use in differentiation studies, cell culture, and functional assays.

Data and images for Human IL-12

Figures

Figure 1

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Human IL-12 activity assay.
The biological activity of Human IL-12 is determined by induction of IFN-γ secretion by PHA-activated T cells.

Figure 1

Human IL-12 activity assay.
The biological activity of Human IL-12 is determined by induction of IFN-γ secretion by PHA-activated T cells.

Figure 2

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SDS-PAGE of Human IL-12, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 2

SDS-PAGE of Human IL-12, premium grade
under reduced (R) and non-reduced (NR) conditions.

Specifications for Human IL-12

Overview

Recombinant human IL-12 (interleukin 12) can promote proliferation of NK and T cells, regulates their cytotoxic activity, and can induce Th1 differentiation of T cells. The T cell-stimulating factor IL-12 is a crucial regulator of cell mediated immune responses as it also induces the production of IFN-γ by PBMCs, among other cytokines. The recombinant protein is manufactured for use in differentiation studies, cell culture, and functional assays.

Applications

Human IL-12 can be used for a variety of applications, including:
  • In vitro differentiation of naive CD4+ T cells towards TH1 cells.
  • In vitro proliferation and stimulation of cytotoxic activity of NK cells and T cells.

Detailed product information

Background information

Interleukin 12 (IL-12) is a heterodimeric proinflammatory cytokine and a modulator of cell-mediated immunity, which is mainly produced by macrophages, dendritic cells, and B cells. IL-12 stimulates the production and secretion of several cytokines, in particular IFN-γ, by NK cells and T cells, induces proliferation, and enhances the cytotoxic activity within these cell populations. Another important activity of IL-12, acting together with IFN-γ and IL-2, is to drive T helper cell responses toward the T
H
1 rather than the T
H
2 phenotype. Moreover, IL-12 is also important in resistance to viral disease and has significant antitumor activity. It has been shown that single chain fusion proteins of naturally occurring heterodimeric cytokines such as IL-12 or IL-23 are bioactive
in vitro
and
in vivo
.
2,3

Biological activity

  • Induction of IFN-γ secretion by human T cells (NIBSC 95/544)
  • premium grade: ≥ 3×
    10
    6
    U/mg
  • We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity, exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www.miltenyibiotec.com/certificates).

Resources for Human IL-12

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Human IL-12

Publications

  1. Wiegand, S. B. et al. (2019) Soluble immune markers in the different phases of chronic hepatitis B virus infection Sci Rep 9(1): 14118
  2. Frumento, G. et al. (2019) CD117 (c-Kit) Is Expressed During CD8 + T Cell Priming and Stratifies Sensitivity to Apoptosis According to Strength of TCR Engagement. Front Immunol 10: 468
  3. Polito, V. A. et al. (2019) Universal Ready-to-Use Immunotherapeutic Approach for the Treatment of Cancer: Expanded and Activated Polyclonal γδ Memory T Cells. Front Immunol 10: 2717
  4. Schurich, A. et al. (2016) Distinct Metabolic Requirements of Exhausted and Functional Virus-Specific CD8 T Cells in the Same Host. Cell Rep 16(5): 1243-1252
  5. Chiurchiù, V. et al. (2016) Proresolving lipid mediators resolvin D1, resolvin D2, and maresin 1 are critical in modulating T cell responses Sci Transl Med 8(353): 353ra111
  6. Bastien, J.-P. et al. (2020) Closing the system: production of viral antigen-presenting dendritic cells eliciting specific CD8 + T cell activation in fluorinated ethylene propylene cell culture bags. J. Transl. Med. 18(1): 383
  7. Klasen, C. et al. (2019) Prostaglandin receptor EP4 expression by Th17 cells is associated with high disease activity in ankylosing spondylitis. Arthritis Res Ther. 21(1): 159
  8. Gajdasik, D. W. et al. (2020) Th1 responses in vivo require cell-specific provision of OX40L dictated by environmental cues. Nat Commun. 11(1): 3421
  9. Loyal, L. et al. (2020) SLAMF7 and IL-6R define distinct cytotoxic versus helper memory CD8 + T cells. Nat Commun. 11(1): 6357
  10. Rossi, G. R. et al. (2020) The Antitumor Effect of Heparin is not Mediated by Direct NK Cell Activation. J Clin Med 9(8): 2666
  11. Wulff, H. et al. (2007) Cloning and characterization of an adenoviral vector for highly efficient and doxycycline-suppressible expression of bioactive human single-chain interleukin 12 in colon cancer. BMC Biotechnol. 7: 35
  12. Lieschke, G. J. et al. (1997)
    Bioactive murine and human interleukin-12 fusion proteins which retain antitumor activity
    in vivo.
    Nat. Biotechnol. 15: 35-40
  13. Miller, J. M. et al. (2010)
    Vesicular stomatitis virus modified with single chain IL-23 exhibits oncolytic activity against tumor cells
    in vitro
    and
    in vivo.
    Int. J. Infereron Cytokine Mediator Res. 2010: 63-72
  14. Juelke, K. et al. (2010)
    CD62L expression identifies a unique subset of polyfunctional CD56
    dim
    NK cells.
    Blood 116(8): 1299-1307

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