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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
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Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies as well as with CD45 and CD15 antibodies. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. The cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. A pre-gate of CD45 +/CD15 – cells was used. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems. |
Figure 1Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies as well as with CD45 and CD15 antibodies. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. The cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. A pre-gate of CD45 +/CD15 – cells was used. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems. | Figure 1Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies as well as with CD45 and CD15 antibodies. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. The cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. A pre-gate of CD45 +/CD15 – cells was used. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems. |
Figure 1Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies as well as with CD45 and CD15 antibodies. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. The cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. A pre-gate of CD45 +/CD15 – cells was used. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems. | Figure 1Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies as well as with CD45 and CD15 antibodies. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. The cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. A pre-gate of CD45 +/CD15 – cells was used. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems. |
Figure 1Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies as well as with CD45 and CD15 antibodies. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. The cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. A pre-gate of CD45 +/CD15 – cells was used. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems. | Figure 1Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies as well as with CD45 and CD15 antibodies. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. The cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. A pre-gate of CD45 +/CD15 – cells was used. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems. |
Figure 1Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies as well as with CD45 and CD15 antibodies. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. The cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. A pre-gate of CD45 +/CD15 – cells was used. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems. | Figure 1Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies as well as with CD45 and CD15 antibodies. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. The cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. A pre-gate of CD45 +/CD15 – cells was used. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems. |
Clone | AC136 |
---|---|
Clonality | monoclonal |
Isotype | mouse IgG2aκ, mouse IgG2a |
Isotype control | Isotype Control Antibody, mouse IgG2a |
Host | mouse |
Type of antibody | Primary antibodies |
Species | human |
Antigen | CD34 |
Alternative names of antigen | gp105-120, Mucosialin, My10 |
Molecular mass of antigen [kDa] | 37 |
Distribution of antigen | stem cells, chondrocytes, dendritic cells, endothelial cells, fibroblasts, mast cells, osteoblasts, cancer stem cells, CNS cells, hematopoietic stem and progenitor cells, bone marrow, liver, dendritic cells, endothelial cells, fibroblasts, osteoblasts, mast cells, stem cells, cancer stem cells, hematopoietic stem and progenitor cells, bone marrow, liver |
Entrez Gene ID | 947 |
RRID | AB_2726005, AB_2726281, AB_2726006, AB_2726278, AB_2726003, AB_2726283, AB_2726008, AB_2726284, AB_2726009, AB_2733225, AB_2733226, AB_2726282, AB_2726007, AB_2726279, AB_2726004, AB_2660378, AB_2726280 |
Will buy and use it again. Recommendable!
Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies as well as with CD45 and CD15 antibodies. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. The cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. A pre-gate of CD45 +/CD15 – cells was used. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems. |
Figure 1Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies as well as with CD45 and CD15 antibodies. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. The cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. A pre-gate of CD45 +/CD15 – cells was used. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems. | Figure 1Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies as well as with CD45 and CD15 antibodies. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. The cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. A pre-gate of CD45 +/CD15 – cells was used. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems. |
Figure 1Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies as well as with CD45 and CD15 antibodies. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. The cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. A pre-gate of CD45 +/CD15 – cells was used. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems. | Figure 1Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies as well as with CD45 and CD15 antibodies. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. The cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. A pre-gate of CD45 +/CD15 – cells was used. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems. |
Figure 1Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies as well as with CD45 and CD15 antibodies. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. The cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. A pre-gate of CD45 +/CD15 – cells was used. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems. | Figure 1Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies as well as with CD45 and CD15 antibodies. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. The cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. A pre-gate of CD45 +/CD15 – cells was used. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems. |
Figure 1Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies as well as with CD45 and CD15 antibodies. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. The cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. A pre-gate of CD45 +/CD15 – cells was used. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems. | Figure 1Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies as well as with CD45 and CD15 antibodies. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. The cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. A pre-gate of CD45 +/CD15 – cells was used. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems. |
Clone | AC136 |
---|---|
Clonality | monoclonal |
Isotype | mouse IgG2aκ, mouse IgG2a |
Isotype control | Isotype Control Antibody, mouse IgG2a |
Host | mouse |
Type of antibody | Primary antibodies |
Species | human |
Antigen | CD34 |
Alternative names of antigen | gp105-120, Mucosialin, My10 |
Molecular mass of antigen [kDa] | 37 |
Distribution of antigen | stem cells, chondrocytes, dendritic cells, endothelial cells, fibroblasts, mast cells, osteoblasts, cancer stem cells, CNS cells, hematopoietic stem and progenitor cells, bone marrow, liver, dendritic cells, endothelial cells, fibroblasts, osteoblasts, mast cells, stem cells, cancer stem cells, hematopoietic stem and progenitor cells, bone marrow, liver |
Entrez Gene ID | 947 |
RRID | AB_2726005, AB_2726281, AB_2726006, AB_2726278, AB_2726003, AB_2726283, AB_2726008, AB_2726284, AB_2726009, AB_2733225, AB_2733226, AB_2726282, AB_2726007, AB_2726279, AB_2726004, AB_2660378, AB_2726280 |
Will buy and use it again. Recommendable!
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