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Plasma cells, through their ability to produce specific antibodies, are essential to control invading viruses and play a primary role in the adaptive immune defense. Isolation of plasma cells from virus-infected individuals can serve as a first step towards the identification of virus-specific antibodies.
The isolation procedure is fast with minimized hands-on time; it can be automated and does not require prior density gradient centrifugation and erythrocyte lysis.
Separation of CD138+ cells from a whole blood sample spiked with U266 cells, using the StraightFrom® Whole Blood and Bone Marrow CD138 MicroBeads. Cells were stained with CD45-FITC, CD138-PE, and CD19-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris, dead cells, and CD45– cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
|Cell type||Product||Order No|
|B cells straight from whole blood||StraightFrom Whole Blood CD19 MicroBeads, human||130-090-880|
|StraightFrom Whole Blood CD138 MicroBeads, human||130-093-062|
|Plasma cells||CD38 MicroBead Kit, human||130-092-263|
|Plasma Cell Isolation Kit II, human||130-093-628|
|CD138 MicroBeads, human||130-051-301|
|Memory B cells||Memory B Cell Isolation Kit, human||130-093-546|
|IgM+ Memory B Cell Isolation Kit, human||130-093-619|
|IgG+ Memory B Cell Isolation Kit, human||130-094-350|
|Total B cells||Pan B Cell Isolation Kit, human||130-101-638|
|CD19 MicroBeads, human||130-050-301|
|CD20 MicroBeads, human||130-091-104|
|REAlease CD19 MicroBead Kit, human||130-117-034|
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