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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
A complete workflow for reliable and efficient T helper cell (TH cell) differentiation, starting with cell isolation of naïve CD4+ T cells and in vitro activation and differentiation, through to comprehensive cell analysis.
Miltenyi Biotec provides gentle cell separation strategies, as well as reliable cell culture reagents such as T cell activation tools, T cell media and polarizing cytokines for the efficient differentiation of TH1, TH2 and TH17 cells.
Discover our T helper cell differentiation workflows
Application data by workflow step
T cell isolation
Isolation of naïve CD4+ T cells
Choose from a variety of MicroBeads and T cell Isolation kits for the separation of human and mouse naïve CD4+ T helper cells.
The Naïve T Cell Isolation Kits were developed for the isolation of untouched naïve T cells from human peripheral blood or mouse spleen single cell suspensions. Naïve T cells are ready for downstream applications, e.g., in studies on naive T helper cell activation and differentiation as well as and in functional studies of DCs.
Isolation of untouched naive CD4+ T helper cells from PBMCs using the Naive CD4+ T Cell Isolation Kit II, human. Cells were stained with CD4 and CD45RA antibodies and analyzed by flow cytometry using the MACSQuant® Analyzer.
Naïve CD4+ T cells were isolated from a single-cell suspension from mouse spleen using the Naive CD4+ T Cell Isolation Kit, mouse. Cells were fluorescently stained with CD45-VioGreen, CD4-VioBlue®, CD62L-PE, CD3ε-APC-Vio770™ , and CD44-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence and a gate was set on CD45+ CD3e+ CD4+ cells.
CD62L, CD45RA and CD45RO MicroBeads
Combine pre-enrichment with the CD4+ T cell Isolation Kits by positive selection or depletion with the respective MicroBeads to isolate even the rarest naïve CD4+ T cell populations.
T cell culture
T cell media
TexMACS™ Medium is an optimized, serum-free cell culture medium, developed for reliable T cell and Treg cell culture results. Chemically-defined and without animal-derived components, it allows for the highest reproducibility in human and mouse cell culture applications.
T cell activation
We offer a variety of polyclonal T cell stimulation tools for you to choose from. Find the options below:
We provide a broad selection of superior-quality cytokines, with high biological activity at a competitive price. Three different grades, research, premium, and MACS® GMP grade, provide flexibility in your assay setup.
Detection and enrichment of cytokine-producing T cells
Cytokine secretion assays are designed for highly sensitive detection and magnetic enrichment of viable cytokine-secreting cells. This unique technology enables detection of one antigen-specific T cell per million cells. Isolated cells can be immediately used for downstream applications, e.g., cell culture and flow cytometric analysis.
A range of kits are available for human and mouse cells secreting TNF-α, GMCSF, IFN-α, IFN-ƴ, or IL-2, -4, -5, -10, -12, -13, -17, -22.
Multiparameter flow analysis of T cells
As the final step in most workflows, flow analysis plays an important part in various T cell applications. Our flow reagents can help you ensure that the data you generate on your flow cytometer is reliable and consistent:
Build your own T cell flow panel with our Flow Panel Builder.
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