Mesenchymal stem cells (MSCs) for cell therapy

  • Complete workflow from clinical-scale MSC isolation to GMP-compliant MSC expansion and cultivation
  • Convenient cell processing with the CliniMACS® System and MACS® GMP Products

Application data by workflow step

Clinical-scale isolation of MSCs

MSCs have been investigated in a variety of clinical trials for the treatment of graft-versus-host disease and tissue regeneration. The antigen CD271 is expressed on MSCs and can be used for the magnetic enrichment of these cells. The characterization of CD271+ cells revealed that the colony-forming-unit fibroblast (CFU-F) potential is highly and exclusively enriched in the CD271+ cell fraction after enrichment using the CliniMACS® System. Following this strategy a well-defined cell population can be used either directly or expanded in culture for pre-clinical and clinical investigations. In addition, the cross-reactivity of the antibody shown in large animals may one day allow for an easy translation of pre-clinical investigations into the clinical area.

CliniMACS CD271 MicroBeads

  • Enrichment of well-defined mesenchymal stromal cells for pre-clinical and clinical investigations
  • Isolated CD271+ cells are highly enriched for CFU-F activity
  • Cells can be directly used or culture and efficiently expanded for further applications
  • Antibody cross-reactivity shown in large animals
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Scientific report
Clinical-scale isolation of mesenchymal stromal cells from bone marrow
according to their CD271 expression

Kathrin Godthardt and Jürgen Schmitz, Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany

GMP-compliant culture of MSCs

MSC-Brew GMP Medium is an optimized and standardized medium for the generation and expansion of MSCs from human bone marrow or other tissue sources. The formulation of MSC-Brew GMP Medium is designed to efficiently support the expansion of MSCs in vitro, while maintaining their differentiation potential. The medium is xeno- and serum-free and is manufactured under strictly controlled conditions using ingredients of the highest quality. MSC-Brew GMP Medium offers consistent lot-to-lot performance and optimal conditions for the cultivation of MSCs.

The poster below shows:

  • MSC-Brew GMP Medium supports the attachment and expansion of MSCs from primary tissue without coating of cell culture vessels.
  • MSC-Brew GMP Medium supports efficient expansion of bona fide MSCs at a clinically relevant scale suited for cell therapy and preserves an immunomodulatory phenotype.
  • MSCs retain their immunosuppressive potential when using GMP-compliant freezing conditions. 
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Scientific poster
Improved clinical-scale MSC isolation from primary tissue and expansion with a GMP matrix- and xeno-free medium

Kathrin Godthardt, Claudia Schreiner, Katharina Freese, Silke Schult, Thomas D. Rockel, Andreas Bosio, and Sebastian Knöbel, Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany

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